Amplification of Herpes Simplex Virus Type 1 DNA in Human Geniculate Ganglia from Formalin‐Fixed, Nonembedded Temporal Bones

Otolaryngology - Head and Neck Surgery - Tập 123 - Trang 508-511 - 2000
Marcos Carreño1, Marı́a Oña1, Santiago Melón1, Jose Luis Llorente1, Juan José Dı́az1, Carlos Suárez1
1From the Departments of Otolaryngology (Drs Carreño, Llorente, Dı́az, and Suárez) and Microbiology (Drs Oña and Melón), Hospital Central de Asturias, Universidad de Oviedo, Spain.

Tóm tắt

Polymerase chain reaction (PCR) has provided new insights in molecular biology. Recently, some studies have been focused on temporal bone pathology, with amplification of DNA from fixed sections of celloidin‐embedded bones. The purpose of our study was to elucidate the utility of PCR in detection of minor concentrations of DNA from nonoptimal stored samples. We obtained geniculate ganglia from 30 temporal bones preserved in formalin for a long time, without any process of embedding. By performing a nested PCR assay, we detected herpes simplex virus type 1 DNA in 13 of 30 ganglia (43%). We conclude therefore that study of temporal bones stored under poor conditions by PCR is possible, although there are some limitations when compared with fresh or optimally archived samples. (Otolaryngol Head Neck Surg 2000;123:508‐11.)

Tài liệu tham khảo

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Thông tin xuất bản

Otolaryngology - Head and Neck Surgery

Tập 123

508-511

Thông tin tác giả