Altered expression of microRNA miR‐21, miR‐155, and let‐7a and their roles in pulmonary neuroendocrine tumors

Pathology International - Tập 62 Số 9 - Trang 583-591 - 2012
Hyoun Wook Lee1, Eun Hee Lee2, Seung Yeon Ha3, Chang Hun Lee4, Hee Kyung Chang5, Sunhee Chang6, Kun Young Kwon7, Ilseon Hwang7, Mee Sook Roh8, Jeong‐Wook Seo9
1Departments of Pathology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Korea.
2Departments of Pathology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon
3Gachon University of Medicine and Science, Incheon
4Busan National University School of Medicine, Busan
5Kosin University College of Medicine, Busan,
6Inje University Ilsan Paik Hospital, Ilsan
7Keimyung University College of Medicine, Daegu
8Dong‐A University College of Medicine, Busan
9Prevention and Management Center, Regional Cardiocerebrovascular Center, Dong‐A University Medical Center, Busan, Korea

Tóm tắt

MicroRNA (miRNA) has a critical effect on tumorigenesis through post‐transcriptional modification and is considered to be potential biomarkers for cancer diagnosis and treatment monitoring. We evaluated the expression pattern of three selected miRNAs (miR‐21, miR‐155, and let‐7a) to evaluate their potential roles by quantitative reverse transcription‐polymerase chain reaction using formalin‐fixed and paraffin‐embedded tissues of 63 surgically resected pulmonary neuroendocrine (NE) tumors (19 typical carcinoids (TCs), 6 atypical carcinoids (ACs), 19 large cell NE carcinomas (LCNECs), and 19 small cell lung carcinomas (SCLCs). Control amplification for U6 small nuclear RNA (U6) was performed in all samples. Normalized Ct values were calculated (CtExperimental miRNA‐CtU6) for each case and recorded. The expression levels of miR‐21 and miR‐155 were significantly higher in high‐grade NE carcinomas (LCNECs and SCLCs) than in carcinoid tumors (TCs and ACs) (each P < 0.001). The expression level of miR‐21 in carcinoid tumors with lymph node metastasis was significantly higher than in carcinoid tumors without lymph node metastasis (P= 0.010). To the best of our knowledge, the present study is the first to examine the expression patterns of miR‐21 and miR‐155 as an adjunctive diagnostic tool or clinically relevant biomarkers for pulmonary NE tumors.

Từ khóa


Tài liệu tham khảo

10.1016/S0959-8049(09)70040-1

Travis WD, 2004, World Health Organization International Histological Classification of Tumours. Pathology and Genetics of Tumors of the Lung, Pleura, Thymus and Heart

10.1111/j.1365-2559.2010.03486.x

10.1016/S0046-8177(98)90047-8

10.1261/rna.642907

10.1016/S0092-8674(04)00045-5

10.1038/nm0705-712

10.1126/science.1064921

10.1089/dna.2006.0544

10.1007/s00428-007-0532-2

10.1038/nrc1840

10.1016/j.ccr.2006.01.025

10.1371/journal.pone.0021300

10.1016/j.ccr.2007.12.008

10.1373/clinchem.2007.101741

10.1073/pnas.0510565103

10.1158/0008-5472.CAN-04-0637

10.4132/KoreanJPathol.2010.44.3.267

10.4132/KoreanJPathol.2012.46.1.42

10.1053/hupa.2000.19294

10.1097/00000478-199808000-00003

10.5761/atcs.oa.08.01309

10.1158/0008-5472.CAN-05-0137

10.1016/j.ajhg.2008.04.005

10.1074/jbc.M611393200

10.1038/modpathol.2011.142

10.1128/MCB.00941-08

10.1038/sj.bjc.6604883

10.1016/j.cell.2005.01.014

Thông tin
Thông tin xuất bản

Pathology International

Tập 62 Số 9

583-591

Thông tin tác giả