Phản ứng lipid thay đổi ở chuột lang ăn hỗn hợp axit linoleic liên hợp cis-9,trans-11+trans-8,cis-10

Lipids - Tập 43 - Trang 251-258 - 2007
Vishnee Bissonauth1, P. Yvan Chouinard2, Johanne Marin1, Nadine Leblanc1, Denis Richard3, Hélène Jacques1
1Department of Food Science and Nutrition and Institute of Nutraceuticals and Functional Foods, Laval University, Quebec, Canada
2Department of Animal Sciences and Institute of Nutraceuticals and Functional Foods, Laval University, Quebec, Canada
3Department of Anatomy and Physiology, Laval University, Quebec, Canada

Tóm tắt

Mục tiêu của nghiên cứu hiện tại là so sánh tác động của hỗn hợp axit linoleic liên hợp cis-9,trans-11 + trans-8,cis-10 (CLA) với hỗn hợp axit linoleic liên hợp cis-9,trans-11 + trans-10,cis-12 CLA và axit linoleic (LA) đối với hồ sơ lipoprotein, lipid gan, thành phần cơ thể và khả năng tiêu hóa chất béo trong chế độ ăn ở chuột lang (n = 17) được cho ăn các chế độ ăn chứa 2% chất béo thí nghiệm (w/w) trong 28 ngày. Hỗn hợp axit CLA cis-9,trans-11 + trans-10,cis-12 cho thấy nồng độ cholesterol LDL cao hơn so với LA và hỗn hợp axit CLA cis-9,trans-11 + trans-8,cis-10. Hỗn hợp axit CLA cis-9,trans-11 + trans-8,cis-10 đã kích thích nồng độ cholesterol LDL huyết tương và lipid gan tương tự như LA, và hệ số khả năng tiêu hóa, cho thấy không có ảnh hưởng của isomer trans-8,cis-10 CLA đến các tham số lipid này. Mặt khác, hỗn hợp axit CLA cis-9,trans-11 + trans-8,cis-10 đã kích thích nồng độ cholesterol VLDL huyết tương và triglycerid cao hơn so với LA và hỗn hợp axit CLA cis-9,trans-11 + trans-10,cis-12. Hỗn hợp axit CLA cis-9,trans-11 + trans-8,cis-10 cũng đã kích thích nồng độ glucose huyết tương cao nhất so với hai nhóm còn lại, cho thấy sự suy giảm trong kiểm soát glycemic. Không có sự khác biệt nào trong thành phần cơ thể giữa ba nhóm. Kết quả hiện tại cho thấy rằng hỗn hợp axit CLA cis-9,trans-11 + trans-8,cis-10 có thể làm suy giảm cholesterol VLDL huyết tương và triglycerid ở chuột lang, có thể do sự tăng cường dòng chảy glucose.

Từ khóa


Tài liệu tham khảo

Kelly GS (2001) Conjugated linoleic acid: a review. Altern Med Rev 6:367–382 Eulitz K, Yurawecz MP, Sehat N, Fritsche J, Roach JA, Mossoba MM, Kramer JK, Adlof RO, Ku Y (1999) Preparation, separation, and confirmation of the eight geometrical cis/trans conjugated linoleic acid isomers 8, 10- through 11, 13 18:2. Lipids 34:873–877 McLeod RS, LeBlanc AM, Langille MA, Mitchell PL, Currie DL (2004) Conjugated linoleic acids, atherosclerosis, and hepatic very-low-density lipoprotein metabolism. Am J Clin Nutr 79:1169S–1174S Destaillats F, Japiot C, Chouinard PY, Arul J, Angers P (2005) Rearrangement of rumenic acid in ruminant fats: a marker of thermal treatment. J Dairy Sci 88:1631–1635 Wang YM, Nagao K, Inoue N, Ujino Y, Shimada Y, Nagao T, Iwata T, Kamegai T, Yamauchi-Sato Y, Yanagita T (2006) Isomer-specific anti-obese and hypolipidemic properties of conjugated linoleic acid in obese OLETF rats. Biosci Biotechnol Biochem 70:355–362 Navarro V, Zabala A, Macarulla MT, Fernandez-Quintela A, Rodriguez VM, Simon E, Portillo MP (2003) Effects of conjugated linoleic acid on body fat accumulation and serum lipids in hamsters fed an atherogenic diet. J Physiol Biochem 59:193–199 Faulconnier Y, Arnal MA, Patureau Mirand P, Chardigny JM, Chilliard Y (2004) Isomers of conjugated linoleic acid decrease plasma lipids and stimulate adipose tissue lipogenesis without changing adipose weight in post-prandial adult sedentary or trained wistar rats. J Nutr Biochem 15:741–748 Nicolosi RJ, Rogers EJ, Kritchevsky D, Scimeca JA, Huth PJ (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherogenesis in hypercholesterolemic hamsters. Artery 22:266–277 Gavino VC, Gavino G, Leblanc MJ, Tuchweber B (2000) An isomeric mixture of conjugated linoleic acids but not pure cis-9, trans-11-octadecadienoic acid affects body weight gain and plasma lipids in hamsters. J Nutr 130:27–29 Kritchevsky D, Tepper SA, Wright S, Tso P, Czarnecki SK (2000) Influence of conjugated linoleic acid (CLA) on establishment and progression of atherosclerosis in rabbits. J Am Coll Nutr 19:472S–477S DeLany JP, Blohm F, Truett AA, Scimeca JA, West DB (1999) Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Am J Physiol 276:R1172–R1179 Park Y, Albright KJ, Liu W, Storkson JM, Cook ME, Pariza MW (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853–858 Larsen TM, Toubro S, Astrup A (2003) Efficacy and safety of dietary supplements containing CLA for the treatment of obesity: evidence from animal and human studies. J Lipid Res 44:2234–2241 De Deckere EA, van Amelsvoort JM, McNeil GP, Jones P (1999) Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. Br J Nutr 82:309–317 Park Y, Storkson JM, Albright KJ, Liu W, Pariza MW (1999) Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids 34:235–241 Nagao K, Wang YM, Inoue N, Han SY, Buang Y, Noda T, Kouda N, Okamatsu H, Yanagita T (2003) The 10trans, 12cis isomer of conjugated linoleic acid promotes energy metabolism in OLETF rats. Nutrition 19:652–656 Bissonauth V, Chouinard Y, Marin J, Leblanc N, Richard D, Jacques H (2006) The effects of t10, c12 CLA isomer compared with c9, t11 CLA isomer on lipid metabolism and body composition in hamsters. J Nutr Biochem 17:597–603 Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951 Hatch FT, Lees RS (1968) Practical methods for plasma lipoprotein analysis. Adv Lipid Res 6:1–68 Folch JM, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509 Richterich R, Dauwalder H (1971) Zur Bertimmung der Plasmaglucokonzentration mit der Hexokinase-Glucose-6-Phosphat-Dehydrogenase Methode. Schweiz Med Wochenschr 101:615–618 Desbuquois B, Aurbach GD (1971) Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J Clin Endocr Metab 37:732–738 Association of Official Analytical Chemists (1984) Official methods of analysis. 14th edn. AOAC, Washington Banni S (2002) Conjugated linoleic acid metabolism. Curr Opin Lipidol 13:261–266 Terpstra AH, Beynen AC, Everts H, Kocsis S, Katan MB, Zock PL (2002) The decrease in body fat in mice fed conjugated linoleic acid is due to increases in energy expenditure and energy loss in the excreta. J Nutr 132:940–945 Yeung CHT, Yang L, Wang J, Chen ZY (2000) Dietary conjugated linoleic acid mixture affects the activity of intestinal acyl coenzyme A: cholesterol acyltransferase in hamsters. Br J Nutr 84:935–941 Wrenn SM Jr, Parks JS, Immermann FW, Rudel L (1995) ACAT inhibitors CL 283,546 and CL 283,796 reduce LDL cholesterol without affecting cholesterol absorption in African green monkeys. J Lipid Res 36:1199–1210 Field KJ, Sibold AL (1999) The laboratory hamster and gerbil. CRC, Boca Raton