Alphavirus vectors and vaccination
Tóm tắt
Alphaviruses are positive‐stranded RNA viruses that have a broad host range and therefore are capable of replicating in many vertebrate and invertebrate cells. The single‐stranded alphavirus genome is divided into two ORFs. The first ORF encodes the nonstructural proteins that are translated upon entry of the virus into the cytoplasm and are responsible for transcription and replication of viral RNA. The second ORF is under the control of a subgenomic promoter and normally encodes the structural proteins, which are responsible for encapsidation of viral RNA and final assembly into enveloped particles. Expression vectors have been engineered from at least three alphaviruses in which the structural protein gene region has been replaced by heterologous genes and have been shown to express high levels of the heterologous protein in cultured cells. These RNA vectors, known as replicons, are capable of replicating on their own but are not packaged into virus‐like particles unless the structural proteins are provided in
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Tài liệu tham khảo
Plotkin SAO, 1999, Vaccines
Schlesinger S, 2001, Fields Virology, 895
McKnight KL, 1996, Deduced consensus sequence of Sindbis virus strain AR339: mutations contained in laboratory strains which affect cell culture and in vivo phenotypes, J Virol, 70, 1981, 10.1128/jvi.70.3.1981-1989.1996
Liljestrom P, 1991, In vitro mutagenesis of a full‐length cDNA clone of Semliki Forest virus: the small 6000‐molecular‐weight membrane protein modulates virus release, J Virol, 65, 4107, 10.1128/jvi.65.8.4107-4113.1991
Frolov IV, 1993, Recombinant equine Venezuelan encephalomyelitis virus, expressing HBsAg, Dokl Akad Nauk, 332, 789
Shirako Y, 2000, Genome structure of Sagiyama virus and its relatedness to other alphaviruses, J Gen Virol, 81, 1353
Hardy WR, 1989, Processing the nonstructural polyproteins of Sindbis virus: nonstructural proteinase is in the C‐terminal half of nsP2 and functions both in cis and in trans, J Virol, 63, 4653, 10.1128/jvi.63.11.4653-4664.1989
LaStarza MW, 1994, Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus‐strand and subgenomic RNA synthesis, J Virol, 68, 5781, 10.1128/jvi.68.9.5781-5791.1994
Hahn YS, 1989, Mapping of RNA‐temperature‐sensitive mutants of Sindbis virus: complementation group F mutants have lesions in nsP4, J Virol, 63, 1194, 10.1128/jvi.63.3.1194-1202.1989
Lemm JA, 1990, Mutations which alter the level or structure of nsP4 can affect the efficiency of Sindbis virus replication in a host‐dependent manner, J Virol, 64, 3001, 10.1128/jvi.64.6.3001-3011.1990
Kobiler D, 1999, A single nucleotide change in the 5′ noncoding region of Sindbis virus confers neurovirulence in rats, J Virol, 73, 10440, 10.1128/JVI.73.12.10440-10446.1999
Hill KR, 1997, RNA–RNA recombination in Sindbis virus: roles of the 3′ conserved motif, poly(A) tail, and nonviral sequences of template RNAs in polymerase recognition and template switching, J Virol, 71, 2693, 10.1128/jvi.71.4.2693-2704.1997
Raju R, 1991, Analysis of Sindbis virus promoter recognition in vivo, using novel vectors with two subgenomic mRNA promoters, J Virol, 65, 2501, 10.1128/jvi.65.5.2501-2510.1991
Levis R, 1990, Promoter for Sindbis virus RNA‐dependent subgenomic RNA transcription, J Virol, 64, 1726, 10.1128/jvi.64.4.1726-1733.1990
Dubuisson J, 1993, Sindbis virus attachment: isolation and characterization of mutants with impaired binding to vertebrate cells, J Virol, 67, 3363, 10.1128/jvi.67.6.3363-3374.1993
Shirako Y, 1994, Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus‐strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus‐ strand RNA synthesis, J Virol, 68, 1874, 10.1128/jvi.68.3.1874-1885.1994
Lemm JA, 1998, Template‐dependent initiation of Sindbis virus RNA replication in vitro, J Virol, 72, 6546, 10.1128/JVI.72.8.6546-6553.1998
Tsiang M, 1985, Studies of defective interfering RNAs of Sindbis virus with and without tRNAAsp sequences at their 5′ termini, J Virol, 54, 38, 10.1128/jvi.54.1.38-44.1985
Rumenapf T, 1994, Subgenomic mRNA of Aura alphavirus is packaged into virions, J Virol, 68, 56, 10.1128/jvi.68.1.56-62.1994
Rumenapf T, 1995, Aura alphavirus subgenomic RNA is packaged into virions of two sizes, J Virol, 69, 1741, 10.1128/jvi.69.3.1741-1746.1995
Kolykhalov AA, 1992, Obtaining infectious Venezuelan equine encephalomyelitis virus based on a full length DNA copy of its genome, Dokl Akad Nauk, 327, 160
Frolov I, 1994, Comparison of the effects of Sindbis virus and Sindbis virus replicons on host cell protein synthesis and cytopathogenicity in BHK cells, J Virol, 68, 1721, 10.1128/jvi.68.3.1721-1727.1994
Ivanova L, 1999, Regulated expression of a Sindbis virus replicon by herpesvirus promoters, J Virol, 73, 1998, 10.1128/JVI.73.3.1998-2005.1999
Polo JM, 1990, Attenuating mutations in glycoproteins E1 and E2 of Sindbis virus produce a highly attenuated strain when combined in vitro, J Virol, 64, 4438, 10.1128/jvi.64.9.4438-4444.1990
Walker DH, 1976, Lymphoreticular and myeloid pathogenesis of Venezuelan equine encephalitis in hamsters, Am J Pathol, 84, 351
Johnston RE, 1996, Fields Virology, 843
Murphy FA, 1970, The role of extraneural arbovirus infection in the pathogenesis of encephalitis. An electron microscopic study of Semliki Forest virus infection in mice, Lab Invest, 22, 318
Velders MP, 2001, Eradication of established tumors by vaccination with Venezuelan equine encephalitis virus replicon particles delivering human papillomavirus 16 E7 RNA, Cancer Res, 61, 7861
Leitner WW, 2000, Enhancement of tumor‐specific immune response with plasmid DNA replicon vectors, Cancer Res, 60, 51
Reap E, 2001, AIDS Vaccine 2001, 2001