Allergenicity Assessment of Genetically-modified Tobacco Expressing Salt Tolerance cbl Gene
Tóm tắt
It is mandatory to assess the allergenic potential of genetically modified (GM) crops before their commercialization. Recently, a transgene [Calcineurin B-like (CBL) protein] has been introduced into tobacco plant to make the crop salt resistance. Therefore, it was felt necessary to assess the allergenic potential of the cbl gene product, which was introduced and expressed in Nicotiana tabacum (tobacco) plant and compared the allergenic effects with the wild-type (WT) counterpart. Bioinformatic analysis revealed that there was no significant sequence homology with known allergens. Also, no difference between the protein digestibility profiles of GM and WT tobacco was found. Rapid digestion of CBL protein (Mol Wt 35 kDa) by simulated gastric fluid (SGF) indicated reduced chances of this protein to induce allergenicity. In addition, BALB/c mice sensitized by intraperitoneal administration of WT and GM tobacco protein showed comparable levels of clinical score, specific IgE, IgG1, histamine level, similar effect on different organs as well as IgE binding proteins. These findings indicate that insertion of cbl gene in tobacco did not cause any additional allergic risk to consumer and the GM and native tobacco proteins behave similarly in both in vitro and in vivo situations even after genetic modification.
Tài liệu tham khảo
Stewart GG (1967) A history of the medicinal use of tobacco. 1492–1860. Med Hist 11:228–268
Golldack D, Li C, Mohan H, Probst N (2014) Tolerance to drought and salt stress in plants: unraveling the signaling networks. Front Plant Sci 5:151
Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics 2014:18
Roy SJ, Negrão S, Tester M (2014) Salt resistant crop plants. Curr Opin Biotechnol 26:115–124
Gilroy S, Trewavas A (2001) Signal processing and transduction in plant cells: the end of the beginning? Nat Rev Mol Cell Biol 2:307–314
Harper JF, Breton G, Harmon A (2004) Decoding Ca (2+) signals through plant protein kinases. Annu Rev Plant Biol 55:263–288
Scrase-Field SA, Knight MR (2003) Calcium: just a chemical switch? Curr Opin Plant Biol 6:500–550
Liu J, Zhu JK (1998) A calcium sensor homolog required for plant salt tolerance. Science 280:1943–1945
Kudla J, Xu Q, Harter K, Gruissem W, Luan S (1999) Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals. Proc Natl Acad Sci U S A 96:4718–4723
Shi J, Kim KN, Ritz O, Albrecht V, Gupta R, Harter K, Luana S, Kudlab J (1999) Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell 11:2393–2405
Hashimoto K, Eckert C, Anschütz U, Scholz M, Held K, Waadt R, Reyer A, Hippler M, Becker D, Kudla J (2012) Phosphorylation of calcineurin B-like (CBL) calcium sensor proteins by their CBL-interacting protein kinases (CIPKs) is required for full activity of CBL-CIPK complexes toward their target proteins. J Biol Chem 287:7956–7968
Kolukisaoglu U, Weinl S, Blazevic D, Batistic O, Kudla J (2004) Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks. Plant Physiol 134:43–58
Metcalfe DD, Astwood JD, Townsend R, Sampson HA, Taylor SL, Fuchs RL (1996) Assessment of the allergenic potential of foods derived from genetically engineered crop plants. Crit Rev Food Sci Nutr 36:S165–S186
Konig A, Cockburn A, Crevel RW, Debruyne E, Grafstroem R, Hammerling U, Kimber I, Knudsen I, Kuiper HA, Peijnenburg AA, Penninks AH, Poulsen M, Schauzu M, Wal JM (2004) Assessment of the safety of foods derived from genetically modified (GM) crops. Food Chem Toxicol 42:1047–1088
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Ballabio C, Magni C, Restani P, Mottini M, Fiocchi A, Tedeschi G, Duranti M (2010) IgE-mediated cross-reactivity among leguminous seed proteins in peanut allergic children. Plant Foods Hum Nutr 65:396–402
Li XM, Schofield BH, Huang CK, Kleiner GI, Sampson HA (1999) A murine model of IgE-mediated cow’s milk hypersensitivity. J Allergy Clin Immunol 103:206–214
Misra A, Kumar R, Mishra V, Chaudhari BP, Raisuddin S, Das M, Dwivedi PD (2011) Potential allergens of green gram (Vigna radiata L. Millsp) identified as members of cupin superfamily and seed albumin. Clin Exp Allergy 41:1157–1168
Misra A, Kumar R, Mishra V, Chaudhari BP, Tripathi A, Das M, Dwivedi PD (2010) Partial characterization of red gram (Cajanus cajan L. Millsp) polypeptides recognized by patients exhibiting rhinitis and bronchial asthma. Food Chem Toxicol 48:2725–2736
Verma AK, Kumar S, Das M, Dwivedi PD (2012) Impact of thermal processing on legume allergens. Plant Foods Hum Nutr 67:430–441
Codex Alimentarius (2009) Foods derived from modern biotechnology. FAO/WHO, Rome, pp 1–85
Astwood JD, Leach JN, Fuchs RL (1996) Stability of food allergens to digestion in vitro. Nat Biotechnol 14:1269–1273
Fu TJ, Upasana RA, Catherine HJ (2002) Digestibility of food allergens and nonallergenic proteins in simulated gastric fluid and simulated intestinal fluids: a comparative study. J Agric Food Chem 50:7154–7160
DBT (2008) Protocols for food and feed safety assessment of GE crops, Department of Biotechnology, Ministry of Science and Technology, Government of India
Kumar S, Verma AK, Misra A, Tripathi A, Chaudhari BP, Prasad R, Jain SK, Das M, Dwivedi PD (2011) Allergenic responses of red kidney bean (Phaseolus vulgaris cv chitra) polypeptides in BALB/c mice recognized by bronchial asthma and allergic rhinitis patients. Food Res Int 44:2868–2879
Katayama S, Mine Y (2006) Quillaja saponin can modulate ovalbumin-induced IgE allergic responses through regulation of Th1/Th2 balance in a murine model. J Agric Food Chem 54:3271–3276
Metzger H (1991) The high affinity receptor for IgE on mast cells. Clin Exp Allergy 21:269–279
O’Byrne PM (1999) Inhaled corticosteroid therapy in newly detected mild asthma. Drugs 58:17–24
Koyasu S (2012) Natural helper cells and TH2-type innate immunity. Cornea 31(Suppl 1):S20–S24. doi:10.1097/ICO.0b013e318269cb1a