Age-related changes in duodenal adaptation after distal small bowel resection in rat
Tóm tắt
Two groups of male Fisher 344 rats (young: 4 months old; aged: 25 months old) underwent either 70% distal small bowel resection or sham operation (small bowel transection). Rats from each treatment group of each age were sacrificed on the 10th (N=15: young rats;N=13: aged rats) or 20th (N=15: young;N=13: aged) postoperative day (POD), and the duodenal mucosa was weighed and assayed for DNA, RNA, and protein contents, as well as for specific activities of the disaccharidase, sucrase, maltase, and lactase. Compared to the sham operation, distal small bowel resection significantly increased DNA by 48%, RNA by 122%, and protein by 75% in young rats and DNA by 40%, RNA by 92%, and protein by 71% in aged rats on the 20th POD. Both young and aged rats showed similar adaptive hyperplasia on the 10th POD. On the 20th POD after distal small bowel resection, specific activities of all tested enzymes were significantly increased in young rats (sucrase +86%, maltase +110% and lactase +64%), but showed no significant changes in aged rats. These findings suggest that the duodenum of aged rats may have sufficient proliferative potential to respond to distal small bowel resection, but that the mechanisms governing return of function in response to distal small bowel resection are inhibited in aged rats, compared to those mechanisms in the young.
Tài liệu tham khảo
Khalil T, Poston GJ, Thompson JC: Effects of aging on gastrointestinal hormones. Prog Clin Biol Res 326:39–71, 1990
Holt PR, Pascal RP, Kotler DP: Effect of aging upon small intestinal structure in the Fisher rat. J Gerontol 39:642–647, 1984
Holt PR, Yeh KY, Kotler DP: Altered controls of proliferation in proximal small intestine of the senescent rat. Proc Natl Acad Sci USA 85:2771–2775, 1988
Holt PR, Yeh KY: Small intestinal crypt cell proliferation rates are increased in senescent rats. J Gerontol 44:B9-B14, 1989
Holt PR, Kotler DP: Adaptive changes of intestinal enzymes to nutritional intake in the aging rat. Gastroenterology 93:295–300, 1987
Bristol JB, Williamson RCN: Postoperative adaptation of the small intestine. World J Surg 9:825–832, 1985
Johnson LR: Regulation of gastrointestinal mucosal growth. Physiol Rev 68:456–502, 1988
Poston GJ, Saydjari R, Lawrence J, Alexander RW, Townsend CM Jr, Thompson JC: The effect of age on small bowel adaptation and growth after proximal enterectomy. J Gerontol 45:B220-B225, 1990
Ogur M, Rosen G: The nucleic acids of plant tissues. I. The extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. Arch Biochem 25:262–276, 1950
Burton K: A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemistry 62:315–323, 1956
Dische Z: Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem 204:983–997, 1953
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951
Dahlqvist A: Assay of intestinal disaccharidase. Anal Biochem 22:99–107, 1968
Dowdy S, Wearden S: Statistics for Research. New York, John Wiley & Sons, 1983
Dowling RH: Small bowel adaptation and its regulation. Scand J Gastroenterol 17 (suppl 74):53–74, 1982
Levine GM: Regulation of intestinal mucosal growth.In Growth of the Gastrointestinal Tract. Gastrointestinal hormones and growth factors. J Morisset, TE Solomon (eds). Boca Raton, Florida, CRC Press, 1991, pp 175–189
Townsend CM Jr, Beauchamp RD, Singh P, Thompson JC: Growth factors and intestinal neoplasms. Am J Surg 155:526–536, 1988
Sagor GR, Ghatei MA, Al-Mukhtar MYT, Wright NA, Bloom SR: Evidence for a humoral mechanism after small intestinal resection. Exclusion of gastrin but not enteroglucagon. Gastroenterology 84:902–906, 1983
Gornacz GE, Al-Mukhtar MYT, Ghatei MA, Sagor GR, Wright NA, Bloom SR: Pattern of cell proliferation and enteroglucagon response following small bowel resection in the rat. Digestion 29:65–72, 1984
Sagor GR, Ghatei MA, O'Shaughnessy DJ, Al-Mukhtar MYT, Wright NA, Bloom SR: Influence of somatostatin and bombesin on plasma enteroglucagon and cell proliferation after intestinal resection in the rat. Gut 26:89–94, 1985
Levine GM, Deren JJ, Yezdimir E: Small-bowel resection oral intake is the stimulus for hyperplasia. Am J Dig Dis 21:542–546, 1976
Holt PR, Tierney AR, Kotler DP: Delayed enzyme expression: A defect of aging rat gut. Gastroenterology 89:1026–1034, 1985
Holt PR, Yeh KY: Aging and small intestinal enzyme activity.In Aging in Liver and Gastrointestinal Tract. L Bianchi, P Holt, OFW James, RN Butler (eds). Lancaster, MTP Press, 1988, pp 69–79
Rosamond W, Savaiano DA: Maintenance of sucrase activity in rat small intestine. Influence of diet and age. Dig Dis Sci 33:1397–1402, 1988
Raul F, Gosse F, Doffoel M, Darmenton P, Wessely JY: Age related increase of brush border enzyme activities along the small intestine. Gut 29:1557–1563, 1988
Williamson RCN: Intestinal adaptation: Structural, functional and cytokinetic changes. N Engl J Med 298:1393–1450, 1978
Weser E, Hernandez MH: Studies of small bowel adaptation after intestinal resection in the rat. Gastroenterology 60:69–75, 1971
McCarthy DM, Kim YS: Changes in sucrase, enterokinase, and peptide hydrolase after intestinal resection. The association of cellular hyperplasia and adaptation. J Clin Invest 52:942–951, 1973
Wilson HD, Miller T, Ogesen B, Schedl HP, Failla ML, Loven DP: Adaptation of the duodenum and ileum of the rat to mid-gut resection: Enzyme activity and trace metal status. Am J Clin Nutr 43:185–193, 1986
Koruda MJ, Rolandelli RH, Settle RG, Rombeau JL: Small bowel disaccharidase activity in the rat as affected by intestinal resection and pectin feeding. Am J Clin Nutr 47:448–453, 1988
Bury KD: Carbohydrate digestion and absorption after massive resection of the small intestine. Surg Gynecol Obstet 135:177–187, 1972
Menge H, Grafe M, Lorenz-Meyer H, Riecken EO: The influence of food intake on the development of structural and functional adaptation following ileal resection in the rat. Gut 16:468–472, 1975
Bochenek WJ, Narczewska B, Grzebieluch M: Effect of massive proximal bowel resection on intestinal sucrase and lactase activity in the rat. Digestion 9:224–230, 1973
Hart MH, Phares CK, Erdnman SH, Grandjean CJ, Park JHY, Vanderhoof JA: Augmentation of postresection mucosal hyperplasia by plerocercoid growth factor (PGF) analog of human growth hormone. Dig Dis Sci 32:1275–1280, 1987