Age Does Matter: A Pilot Comparison of Placenta-Derived Stromal Cells for in utero Repair of Myelomeningocele Using a Lamb Model

Fetal Diagnosis and Therapy - Tập 39 Số 3 - Trang 179-185 - 2016
Erin G. Brown1, B Keller1, Lee Lankford1, Christopher D. Pivetti1, Shinjiro Hirose1, Diana L. Farmer1, Aijun Wang1
1Department of Surgery, University of California, Davis Health System, Sacramento, Calif., USA

Tóm tắt

<b><i>Introduction:</i></b> Fetal amniotic membranes (FM) have been shown to preserve spinal cord histology in the fetal sheep model of myelomeningocele (MMC). This study compares the effectiveness of placenta-derived mesenchymal stromal cells (PMSCs) from early-gestation versus term-gestation placenta to augment FM repair to improve distal motor function in a sheep model. <b><i>Methods:</i></b> Fetal lambs (n = 4) underwent surgical MMC creation followed by repair with FM patch with term-gestation PMSCs (n = 1), FM with early-gestation PMSCs (n = 1), FM only (n = 1), and skin closure only (n = 1). Histopathology and motor assessment was performed. <b><i>Results:</i></b> Histopathologic analysis demonstrated increased preservation of spinal cord architecture and large neurons in the lamb repaired with early-gestation cells compared to all others. Lambs repaired with skin closure only, FM alone, and term-gestation PMSCs exhibited extremely limited distal motor function; the lamb repaired with early-gestation PMSCs was capable of normal ambulation. <b><i>Discussion:</i></b> This pilot study is the first in vivo comparison of different gestational-age placenta-derived stromal cells for repair in the fetal sheep MMC model. The preservation of large neurons and markedly improved motor function in the lamb repaired with early-gestation cells suggest that early-gestation placental stromal cells may exhibit unique properties that augment in utero MMC repair to improve paralysis.

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Tài liệu tham khảo

10.1056/NEJMoa1014379

10.1016/j.jpedsurg.2013.09.043

10.12968/jowc.2012.21.6.290

10.1080/09273970802405493

10.1586/eop.10.63

10.1007/s00441-012-1424-6

10.1111/j.1524-475X.2007.00252.x

10.4103/0975-5950.94469

10.1016/j.bbrc.2007.08.033

10.1016/j.intimp.2012.03.024

10.1016/j.ajog.2006.01.101

10.1016/j.jcyt.2013.06.019

10.4252/wjsc.v4.i6.53

10.1016/j.ajog.2005.02.110

10.1016/S0022-3468(97)90603-5

10.1016/j.jpedsurg.2015.01.002

10.1089/neu.2006.23.36

10.1038/nm0495-342

10.1016/j.bpobgyn.2004.07.001

10.1002/uog.14636

10.1002/stem.1757

10.1016/S0896-6273(03)00497-5

10.1016/j.jpedsurg.2014.10.021

10.3727/096368913X667709

10.1016/j.addr.2014.10.007

10.1067/mob.2000.108867