Aerobic and sequential anaerobic fermentation to produce xylitol and ethanol using non-detoxified acid pretreated corncob
Tóm tắt
For economical bioethanol production from lignocellulosic materials, the major technical challenges to lower the production cost are as follows: (1) The microorganism should use efficiently all glucose and xylose in the lignocellulose hydrolysate. (2) The microorganism should have high tolerance to the inhibitors present in the lignocellulose hydrolysate. The aim of the present work was to combine inhibitor degradation, xylitol fermentation, and ethanol production using a single yeast strain. A new process of integrated aerobic xylitol production and anaerobic ethanol fermentation using non-detoxified acid pretreated corncob by Candida tropicalis W103 was proposed. C. tropicalis W103 is able to degrade acetate, furfural, and 5-hydromethylfurfural and metabolite xylose to xylitol under aerobic conditions, and the aerobic fermentation residue was used as the substrate for ethanol production by anaerobic simultaneous saccharification and fermentation. With 20% substrate loading, furfural and 5-hydroxymethylfurfural were degraded totally after 60 h aerobic incubation. A maximal xylitol concentration of 17.1 g l-1 was obtained with a yield of 0.32 g g-1 xylose. Then under anaerobic conditions with the addition of cellulase, 25.3 g l-1 ethanol was produced after 72 h anaerobic fermentation, corresponding to 82% of the theoretical yield. Xylitol and ethanol were produced in Candida tropicalis W103 using dual-phase fermentations, which comprise a changing from aerobic conditions (inhibitor degradation and xylitol production) to anaerobic simultaneous saccharification and ethanol fermentation. This is the first report of integrated xylitol and ethanol production from non-detoxified acid pretreated corncob using a single microorganism.
Tài liệu tham khảo
Qu YB, Zhu MT, Liu K, Bao XM, Lin JQ: Studies on cellulosic ethanol production for sustainable supply of liquid fuel in China. Biotechnol J 2006, 1: 1235-1240. 10.1002/biot.200600067
Cheng KK, Zhang JA, Erik C, Li JP: Integrated production of xylitol and ethanol using corn cob. Appl Microbiol Biotechnol 2010, 87: 411-417. 10.1007/s00253-010-2612-5
Lin TH, Huang CF, Guo GL, Hwang WS, Huang SL: Pilot-scale ethanol production from rice straw hydrolysates using xylose-fermenting Pichia stipitis . Bioresour Technol 2012, 116: 314-319. 10.1016/j.biortech.2012.03.089
Matsushika A, Inoue H, Kodaki T, Sawayama S: Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives. Appl Microbiol Biotechnol 2009, 84: 37-53. 10.1007/s00253-009-2101-x
Wierckx N, Koopman F, Ruijssenaars HJ: Microbial degradation of furanic compounds: biochemistry, genetics, and impact. Appl Microbiol Biotechnol 2011, 92: 1095-1105. 10.1007/s00253-011-3632-5
Boopathy R, Bokang H, Daniels L: Biotransformation of furfural and 5-hydroxymethyl furfural by enteric bacteria. J Ind Microbiol Biotechnol 1993, 11: 147-150.
Saha B, Iten L, Cotta M, Wu V: Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol. Biotechnol Progr 2005, 21: 816-822. 10.1021/bp049564n
Schirmer-Michel AC, Flores SH, Hertz PF, Matos GS, Zachia Ayub MA: Production of ethanol from soybean hull hydrolysate by osmotolerant Candida guilliermondii NRRL Y-2075. Bioresour Technol 2008, 99: 2898-2904. 10.1016/j.biortech.2007.06.042
Schirmer-Michel AC, Flores SH, Hertz PF, Zachia Ayub MA: Effect of oxygen transfer rates on alcohols production by Candida guilliermondii cultivated on soybean hull hydrolysate. J Chem Technol Biotechnol 2009, 84: 223-228. 10.1002/jctb.2028
Varga E, Klinke HB, Reczey K, Thomsen AB: High solid simultaneous saccharification and fermentation of wet oxidized corn stover to ethanol. Biotechnol Bioeng 2004, 88: 567-574. 10.1002/bit.20222
Ping Y, Ling HZ, Song G, Ge JP: Xylitol production from non-detoxified corncob hemicellulose acid hydrolysate by Candida tropicalis . Biochem Eng J 2013, 75: 86-91. 10.1016/j.bej.2013.03.022
Buhner J, Agblevor FA: Effect of detoxification of dilute-acid corn fiber hydrolysate on xylitol production. Appl Biochem Biotech 2004, 119: 13-30. 10.1385/ABAB:119:1:13
Liaw WC, Chen CS, Chang WS, Chen KP: Xylitol production from rice straw hemicellulose hydrolyzate by polyacrylic hydrogel thin films with immobilized Candida subtropicalis WF79. J Biosci Bioeng 2008, 105: 97-105. 10.1263/jbb.105.97
Kiyoshi T, Jun-ichi H, Tohru K, Masayoshi K: Microbial xylitol production from corn cobs using Candida magnoliae . J Biosci Bioeng 2004, 98: 228-230. 10.1016/S1389-1723(04)00273-7
Villarreal MLM, Prata AMR, Felipe MGA, Almeida JBES: Detoxification procedures of eucalyptus hemicellulose hydrolysate for xylitol production by Candida guilliermondii . Enzyme MicrobTechnol 2006, 40: 17-24. 10.1016/j.enzmictec.2005.10.032
Carvalheiro F, Duarte LC, Lopes S, Parajó JC, Pereira H, Gírio FM: Evaluation of the detoxification of brewery’s spent grain hydrolysate for xylitol production by Debaryomyces hansenii CCMI 941. Process Biochem 2005, 40: 1215-1223. 10.1016/j.procbio.2004.04.015
Zhang J, Zhu ZN, Wang XF, Wang N, Wang W, Bao J: Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation. Biotechnol Biofuels 2010, 3: 26. 10.1186/1754-6834-3-26
Huang CF, Jiang YF, Guo GL, Hwang WS: Development of a yeast strain for xylitol production without hydrolysate detoxification as part of the integration of co-product generation within the lignocellulosic ethanol process. Bioresour Technol 2011, 102: 3322-3329. 10.1016/j.biortech.2010.10.111
Almeida J, Modig T, Roder A, Liden G, Gorwa-Grauslund MF: Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF). Biotechnol Biofuels 2008, 1: 12. 10.1186/1754-6834-1-12
Hickert LR, de Souza-Cruz PB, Rosa CA, Ayub MAZ: Simultaneous saccharification and co-fermentation of un-detoxified rice hull hydrolysate by Saccharomyces cerevisiae ICV D254 and Spathaspora arborariae NRRL Y-48658 for the production of ethanol and xylitol. Bioresour Technol 2013, 143: 112-116. 10.1016/j.biortech.2013.05.123
Zhang J, Chu DQ, Huang J, Yu ZC, Dai GC, Bao J: Simultaneous saccharification and ethanol fermentation at high corn stover solids loading in a helical stirring bioreactor. Biotechnol Bioeng 2010, 105: 718-728.
Ghose TK: Measurement of cellulase activities. Pure Appl Chem 1987, 59: 257-268.