Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Chất lỏng điều kiện từ tế bào gốc lấy từ mỡ bảo vệ tế bào nguyên bào sợi ở các mức độ lão hóa khác nhau khỏi tổn thương do bức xạ UVB
Tóm tắt
Tế bào gốc lấy từ mỡ (ADSCs) và các sản phẩm của chúng đã thu hút sự quan tâm mạnh mẽ trong các lĩnh vực y học da liễu và thẩm mỹ. Là một thành phần chính được phát hiện trong bí quyết tiết ra của ADSCs, yếu tố tăng trưởng nguồn gốc tiểu cầu AA (PDGF-AA) đã được báo cáo là trung gian trong việc lắng đọng và tái cấu trúc ma trận ngoại bào, do đó có thể góp phần vào hiệu ứng chống lão hóa của nó. Dựa trên việc thiết lập một mô hình thực nghiệm mô phỏng quá trình lão hóa da thực tế bằng cách làm cho các nguyên bào sợi da người (HDFs) tiếp xúc với cả các yếu tố lão hóa nội sinh và ngoại sinh, chúng tôi đã tiền điều trị các HDFs bằng môi trường nuôi cấy điều kiện từ ADSC (ADSC-CM) trước khi bị chiếu xạ, nhằm khám phá tác động ngăn ngừa của bí quyết tiết ra từ ADSCs đối với tổn thương lão hóa. 48 giờ sau khi chiếu xạ, chúng tôi đã phát hiện sự phát triển tế bào; nhuộm β-galactosidase; biểu hiện mRNA của MMP-1, MMP-9 và TIMP-1; và biểu hiện protein của collagen I, collagen III và elastin. Hơn nữa, chúng tôi cũng phát hiện biểu hiện protein liên quan đến con đường tín hiệu PI3K/Akt, có thể được kích hoạt bởi PDGF-AA và mới được phát hiện là thúc đẩy tổng hợp protein ma trận ngoại bào. Nồng độ PDGF-AA trong ADSC-CM đã chuẩn bị giảm theo thời gian và duy trì hoạt tính sinh học tuyệt vời ở nhiệt độ thấp cho đến tuần thứ 11. Tiền điều trị bằng ADSC-CM có thể cải thiện một cách nhẹ nhàng hoặc đáng kể hoạt động tăng trưởng tế bào và giảm sự lão hóa tế bào ở các HDFs đã bị chiếu xạ. Bên cạnh đó, tiền điều trị bằng ADSC-CM cũng làm tăng biểu hiện collagen I, collagen III, elastin và TIMP-1 nhưng giảm biểu hiện MMP-1 và MMP-9 ở cả các HDFs đã bị chiếu xạ và không bị chiếu xạ. Tiền điều trị bằng ADSC-CM đã làm tăng đáng kể biểu hiện protein pAkt, và biểu hiện protein ECM đã giảm mạnh trong trường hợp sử dụng LY294002. Các kết quả tương tự đã được ghi nhận ở ba thế hệ HDFs, nhưng khác nhau ở các mức độ khác nhau. Nhìn chung, ADSC-CM mà chúng tôi chuẩn bị cho thấy một mức độ nào đó vai trò tích cực trong việc ngăn ngừa các HDFs khỏi tổn thương lão hóa nội sinh và ngoại sinh, và rằng PDGF-AA có thể góp phần giúp nó trở nên hiệu quả với một số thành phần khác trong ADSC-CM.
Từ khóa
#tế bào gốc #tế bào nguyên bào sợi #biến đổi lão hóa #PDGF-AA #ma trận ngoại bào #hồi phục daTài liệu tham khảo
Naylor EC, Watson RE, Sherratt MJ (2011) Molecular aspects of skin ageing. Maturitas 69:249–256. https://doi.org/10.1016/j.maturitas.2011.04.011
Warren R, Gartstein V, Kligman AM, Montagna W, Allendorf RA, Ridder GM (1991) Age, sunlight, and facial skin: a histologic and quantitative study. J Am Acad Dermatol 25:751–760
Bailey AJ (2001) Molecular mechanisms of ageing in connective tissues. Mech Ageing Dev 122:735–755
Tashiro K, Shishido M, Fujimoto K, Hirota Y, Yo K, Gomi T, Tanaka Y (2014) Age-related disruption of autophagy in dermal fibroblasts modulates extracellular matrix components. Biochem Biophys Res Commun 443:167–172. https://doi.org/10.1016/j.bbrc.2013.11.066
Wang T, Guo S, Liu X, Xv N, Zhang S (2015) Protective effects of adipose-derived stem cells secretome on human dermal fibroblasts from ageing damages. Int J Clin Exp Pathol 8:15739–15748
Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228. https://doi.org/10.1089/107632701300062859
Miana VV, Gonzalez EAP (2018) Adipose tissue stem cells in regenerative medicine. Ecancermedicalscience 12:822. https://doi.org/10.3332/ecancer.2018.822
Lin HP, Chan TM, Fu RH, Chuu CP, Chiu SC, Tseng YH, Liu SP, Lai KC, Shih MC, Lin ZS, Chen HS, Yeh DC, Lin SZ (2015) Applicability of adipose-derived stem cells in type 1 diabetes mellitus. Cell Transpl 24:521–532. https://doi.org/10.3727/096368915X686977
Chan TM, Chen JY, Ho LI, Lin HP, Hsueh KW, Liu DD, Chen YH, Hsieh AC, Tsai NM, Hueng DY, Tsai ST, Chou PW, Lin SZ, Harn HJ (2014) ADSC therapy in neurodegenerative disorders. Cell Transpl 23:549–557. https://doi.org/10.3727/096368914X678445
Won CH, Park GH, Wu X, Tran TN, Park KY, Park BS, Kim DY, Kwon O, Kim KH (2017) The basic mechanism of hair growth stimulation by adipose-derived stem cells and their secretory factors. Curr Stem Cell Res Ther 12:535–543. https://doi.org/10.2174/1574888X12666170829161058
Kim MH, Wu WH, Choi JH, Kim J, Jun JH, Ko Y, Lee JH (2017) Galectin-1 from conditioned medium of three-dimensional culture of adipose-derived stem cells accelerates migration and proliferation of human keratinocytes and fibroblasts. Wound Repair Regen. https://doi.org/10.1111/wrr.12579
Nakhaeifard M, Haji Ghasem Kashani M, Goudarzi I, Rezaei A (2018) Conditioned medium protects dopaminergic neurons in parkinsonian rats. Cell J 20:348–354. https://doi.org/10.22074/cellj.2018.5343
Jeong JH, Fan Y, You GY, Choi TH, Kim S (2015) Improvement of photoaged skin wrinkles with cultured human fibroblasts and adipose-derived stem cells: a comparative study. J Plast Reconstr Aesthet Surg 68:372–381. https://doi.org/10.1016/j.bjps.2014.10.045
Xu X, Wang HY, Zhang Y, Liu Y, Li YQ, Tao K, Wu CT, Jin JD, Liu XY (2014) Adipose-derived stem cells cooperate with fractional carbon dioxide laser in antagonizing photoaging: a potential role of Wnt and beta-catenin signaling. Cell Biosci 4:24. https://doi.org/10.1186/2045-3701-4-24
Song SY, Jung JE, Jeon YR, Tark KC, Lew DH (2011) Determination of adipose-derived stem cell application on photo-aged fibroblasts, based on paracrine function. Cytotherapy 13:378–384. https://doi.org/10.3109/14653249.2010.530650
Wang X, Shu X, Huo W, Zou L, Li L (2017) Efficacy of protein extracts from medium of Adipose-derived stem cells via microneedles on Asian skin. J Cosmet Laser Ther. https://doi.org/10.1080/14764172.2017.1400171
Gaur M, Dobke M, Lunyak VV (2017) Mesenchymal stem cells from adipose tissue in clinical applications for dermatological indications and skin aging. Int J Mol Sci 18:208. https://doi.org/10.3390/ijms18010208
Zhou BR, Xu Y, Guo SL, Xu Y, Wang Y, Zhu F, Permatasari F, Wu D, Yin ZQ, Luo D (2013) The effect of conditioned media of adipose-derived stem cells on wound healing after ablative fractional carbon dioxide laser resurfacing. Biomed Res Int 2013:519126. https://doi.org/10.1155/2013/519126
Peinado JR, Pardo M, de la Rosa O, Malagon MM (2012) Proteomic characterization of adipose tissue constituents, a necessary step for understanding adipose tissue complexity. Proteomics 12:607–620. https://doi.org/10.1002/pmic.201100355
Kim MS, Song HJ, Lee SH, Lee CK (2014) Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts. J Cosmet Dermatol 13:44–51. https://doi.org/10.1111/jocd.12073
Seikrit C, Henkel C, van Roeyen CR, Bokemeyer D, Eitner F, Martin IV, Boor P, Knuchel R, Meyer HE, Muller-Newen G, Eriksson U, Floege J, Ostendorf T (2013) Biological responses to PDGF-AA versus PDGF-CC in renal fibroblasts. Nephrol Dial Transpl 28:889–900. https://doi.org/10.1093/ndt/gfs509
Yeo EJ, Hwang YC, Kang CM, Kim IH, Kim DI, Parka JS, Choy HE, Park WY, Park SC (2000) Senescence-like changes induced by hydroxyurea in human diploid fibroblasts. Exp Gerontol 35:553–571
Yeo EJ, Hwang YC, Kang CM, Choy HE, Park SC (2000) Reduction of UV-induced cell death in the human senescent fibroblasts. Mol Cells 10:415–422
Shin H, Won CH, Chung WK, Park BS (2017) Up-to-date clinical trials of hair regeneration using conditioned media of adipose-derived stem cells in male and female pattern hair loss. Curr Stem Cell Res Ther 12:524–530. https://doi.org/10.2174/1574888X12666170504120244
Zhang W, Bai X, Zhao B, Li Y, Zhang Y, Li Z, Wang X, Luo L, Han F, Zhang J, Han S, Cai W, Su L, Tao K, Shi J, Hu D (2018) Cell-free therapy based on adipose tissue stem cell-derived exosomes promotes wound healing via the PI3K/Akt signaling pathway. Exp Cell Res 370:333–342. https://doi.org/10.1016/j.yexcr.2018.06.035
Kapur SK, Katz AJ (2013) Review of the adipose derived stem cell secretome. Biochimie 95:2222–2228. https://doi.org/10.1016/j.biochi.2013.06.001
Gross SM, Rotwein P (2016) Unraveling growth factor signaling and cell cycle progression in individual fibroblasts. J Biol Chem 291:14628–14638. https://doi.org/10.1074/jbc.M116.734194
Simm A, Nestler M, Hoppe V (1998) Mitogenic effect of PDGF-AA on cardiac fibroblasts. Basic Res Cardiol 93(Suppl 3):40–43
Yang SY, Xu GM (2001) Expression of PDGF and its receptor as well as their relationship to proliferating activity and apoptosis of meningiomas in human meningiomas. J Clin Neurosci 8(Suppl 1):49–53. https://doi.org/10.1054/jocn.2001.0877
Vantler M, Huntgeburth M, Caglayan E, Ten Freyhaus H, Schnabel P, Rosenkranz S (2006) PI3-kinase/Akt-dependent antiapoptotic signaling by the PDGF alpha receptor is negatively regulated by Src family kinases. FEBS Lett 580:6769–6776. https://doi.org/10.1016/j.febslet.2006.11.034
Donovan J, Shiwen X, Norman J, Abraham D (2013) Platelet-derived growth factor alpha and beta receptors have overlapping functional activities towards fibroblasts. Fibrogenesis Tissue Repair 6:10. https://doi.org/10.1186/1755-1536-6-10
Schneider L, Cammer M, Lehman J, Nielsen SK, Guerra CF, Veland IR, Stock C, Hoffmann EK, Yoder BK, Schwab A, Satir P, Christensen ST (2010) Directional cell migration and chemotaxis in wound healing response to PDGF-AA are coordinated by the primary cilium in fibroblasts. Cell Physiol Biochem 25:279–292. https://doi.org/10.1159/000276562
Rakhila H, Al-Akoum M, Bergeron ME, Leboeuf M, Lemyre M, Akoum A, Pouliot M (2016) Promotion of angiogenesis and proliferation cytokines patterns in peritoneal fluid from women with endometriosis. J Reprod Immunol 116:1–6. https://doi.org/10.1016/j.jri.2016.01.005
Glady A, Tanaka M, Moniaga CS, Yasui M, Hara-Chikuma M (2018) Involvement of NADPH oxidase 1 in UVB-induced cell signaling and cytotoxicity in human keratinocytes. Biochem Biophys Rep 14:7–15. https://doi.org/10.1016/j.bbrep.2018.03.004
Jia Y, Qin Q, Fang CP, Shen W, Sun TT, Huang YL, Li WJ, Deng AM (2018) UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract. Int J Mol Med 41:3041–3050. https://doi.org/10.3892/ijmm.2018.3478
Kim WS, Park BS, Park SH, Kim HK, Sung JH (2009) Antiwrinkle effect of adipose-derived stem cell: activation of dermal fibroblast by secretory factors. J Dermatol Sci 53:96–102. https://doi.org/10.1016/j.jdermsci.2008.08.007
Kim WS, Park BS, Kim HK, Park JS, Kim KJ, Choi JS, Chung SJ, Kim DD, Sung JH (2008) Evidence supporting antioxidant action of adipose-derived stem cells: protection of human dermal fibroblasts from oxidative stress. J Dermatol Sci 49:133–142. https://doi.org/10.1016/j.jdermsci.2007.08.004
Diez C, Nestler M, Friedrich U, Vieth M, Stolte M, Hu K, Hoppe J, Simm A (2001) Down-regulation of Akt/PKB in senescent cardiac fibroblasts impairs PDGF-induced cell proliferation. Cardiovasc Res 49:731–740
Kohl E, Steinbauer J, Landthaler M, Szeimies RM (2011) Skin ageing. J Eur Acad Dermatol Venereol 25:873–884. https://doi.org/10.1111/j.1468-3083.2010.03963.x
Calleja-Agius J, Brincat M, Borg M (2013) Skin connective tissue and ageing. Best Pract Res Clin Obstet Gynaecol 27:727–740. https://doi.org/10.1016/j.bpobgyn.2013.06.004
Vincenti MP, White LA, Schroen DJ, Benbow U, Brinckerhoff CE (1996) Regulating expression of the gene for matrix metalloproteinase-1 (collagenase): mechanisms that control enzyme activity, transcription, and mRNA stability. Crit Rev Eukaryot Gene Expr 6:391–411
Van Doren SR (2015) Matrix metalloproteinase interactions with collagen and elastin. Matrix Biol 44–46:224–231. https://doi.org/10.1016/j.matbio.2015.01.005
Cho JW, Kang MC, Lee KS (2010) TGF-beta1-treated ADSCs-CM promotes expression of type I collagen and MMP-1, migration of human skin fibroblasts, and wound healing in vitro and in vivo. Int J Mol Med 26:901–906
Jung H, Kim HH, Lee DH, Hwang YS, Yang HC, Park JC (2011) Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile. Cytotechnology 63:57–66. https://doi.org/10.1007/s10616-010-9327-4
Kim WS, Park SH, Ahn SJ, Kim HK, Park JS, Lee GY, Kim KJ, Whang KK, Kang SH, Park BS, Sung JH (2008) Whitening effect of adipose-derived stem cells: a critical role of TGF-beta 1. Biol Pharm Bull 31:606–610
Kisand K, Tamm AE, Lintrop M, Tamm AO (2018) New insights into the natural course of knee osteoarthritis: early regulation of cytokines and growth factors, with emphasis on sex-dependent angiogenesis and tissue remodeling. A pilot study. Osteoarthr Cartil 26:1045–1054. https://doi.org/10.1016/j.joca.2018.05.009
Robbins JR, McGuire PG, Wehrle-Haller B, Rogers SL (1999) Diminished matrix metalloproteinase 2 (MMP-2) in ectomesenchyme-derived tissues of the Patch mutant mouse: regulation of MMP-2 by PDGF and effects on mesenchymal cell migration. Dev Biol 212:255–263. https://doi.org/10.1006/dbio.1999.9373
Zhang Q, Lai S, Hou X, Cao W, Zhang Y, Zhang Z (2018) Protective effects of PI3K/Akt signal pathway induced cell autophagy in rat knee joint cartilage injury. Am J Transl Res 10:762–770
Zhang X, Liang D, Lian X, Jiang Y, He H, Liang W, Zhao Y, Chi ZH (2016) Berberine activates Nrf2 nuclear translocation and inhibits apoptosis induced by high glucose in renal tubular epithelial cells through a phosphatidylinositol 3-kinase/Akt-dependent mechanism. Apoptosis 21:721–736. https://doi.org/10.1007/s10495-016-1234-5
Ito M, Yurube T, Kakutani K, Maeno K, Takada T, Terashima Y, Kakiuchi Y, Takeoka Y, Miyazaki S, Kuroda R, Nishida K (2017) Selective interference of mTORC1/RAPTOR protects against human disc cellular apoptosis, senescence, and extracellular matrix catabolism with Akt and autophagy induction. Osteoarthr Cartil 25:2134–2146. https://doi.org/10.1016/j.joca.2017.08.019
Wada Y, Yoshida K, Tsutani Y, Shigematsu H, Oeda M, Sanada Y, Suzuki T, Mizuiri H, Hamai Y, Tanabe K, Ukon K, Hihara J (2007) Neutrophil elastase induces cell proliferation and migration by the release of TGF-alpha, PDGF and VEGF in esophageal cell lines. Oncol Rep 17:161–167
Han J, Jin W, Ho NA, Hong J, Kim YJ, Shin Y, Lee H, Suh JW (2018) Decursin and decursinol angelate improve wound healing by upregulating transcription of genes encoding extracellular matrix remodeling proteins, inflammatory cytokines, and growth factors in human keratinocytes. Biochem Biophys Res Commun 499:979–984. https://doi.org/10.1016/j.bbrc.2018.04.031
Tamariz-Dominguez E, Castro-Munozledo F, Kuri-Harcuch W (2002) Growth factors and extracellular matrix proteins during wound healing promoted with frozen cultured sheets of human epidermal keratinocytes. Cell Tissue Res 307:79–89. https://doi.org/10.1007/s004410100450