Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction

Journal of Translational Medicine - Tập 9 Số 1 - 2011
Yen-Ta Chen1, Cheuk‐Kwan Sun2, Yu‐Chun Lin3, Li-Teh Chang4, Yung‐Lung Chen3, Tzu‐Hsien Tsai3, Sheng-Ying Chung3, Sarah Chua3, Ying-Hsien Kao5, Chia‐Hung Yen6, Pei‐Lin Shao7, Kuan-Cheng Chang8, Steve Leu9, Hon‐Kan Yip9
1Division of Urology, Department of Surgery, Chang Gung Memorial Hospital - Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan
2Department of Emergency Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan
3Division of cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan
4Basic Science, Nursing Department, Meiho University, Pingtung, Taiwan
5Department of Medical Research, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan
6Department of Life Science, National Pingtung University of Science and Technology, Pingtung, Taiwan
7Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
8Department of Cardiology, School of Medicine, China Medical University, Taichung, Taiwan
9Center for Translational Research in Biomedical Sciences, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

Tóm tắt

Abstract Background

Reactive oxygen species are important mediators exerting toxic effects on various organs during ischemia-reperfusion (IR) injury. We hypothesized that adipose-derived mesenchymal stem cells (ADMSCs) protect the kidney against oxidative stress and inflammatory stimuli in rat during renal IR injury.

 Methods

Adult male Sprague-Dawley (SD) rats (n = 24) were equally randomized into group 1 (sham control), group 2 (IR plus culture medium only), and group 3 (IR plus immediate intra-renal administration of 1.0 × 106 autologous ADMSCs, followed by intravenous ADMSCs at 6 h and 24 h after IR). The duration of ischemia was 1 h, followed by 72 hours of reperfusion before the animals were sacrificed.

 Results

Serum creatinine and blood urea nitrogen levels and the degree of histological abnormalities were markedly lower in group 3 than in group 2 (all p < 0.03). The mRNA expressions of inflammatory, oxidative stress, and apoptotic biomarkers were lower, whereas the anti-inflammatory, anti-oxidative, and anti-apoptotic biomarkers were higher in group 3 than in group 2 (all p < 0.03). Immunofluorescent staining showed a higher number of CD31+, von Willebrand Factor+, and heme oxygenase (HO)-1+ cells in group 3 than in group 2 (all p < 0.05). Western blot showed notably higher NAD(P)H quinone oxidoreductase 1 and HO-1 activities, two indicators of anti-oxidative capacity, in group 3 than those in group 2 (all p < 0.04). Immunohistochemical staining showed higher glutathione peroxidase and glutathione reductase activities in group 3 than in group 2 (all p < 0.02)

 Conclusion

ADMSC therapy minimized kidney damage after IR injury through suppressing oxidative stress and inflammatory response.

Từ khóa


Tài liệu tham khảo

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Journal of Translational Medicine

Tập 9 Số 1

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