Adhesive protein‐free synthetic hydrogels for retinal pigment epithelium cell culture with low ROS level

Journal of Biomedical Materials Research - Part A - Tập 102 Số 7 - Trang 2258-2267 - 2014
Yong Mei Chen1,2, Zhen Qi Liu1,2, Zhihui Feng3, Feng Xu1,3, Jian Kang Liu3
1Biomedical Engineering and Biomechanics Center, Xi'an Jiaotong University, Xi'an, 710049 People's Republic of China
2Department of Chemistry, School of Science, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an, 710049 People's Republic of China
3Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Life Science, FIST, Xi'an Jiaotong University, Xi'an, 710049 People's Republic of China

Tóm tắt

AbstractEngineering of human retinal pigment epithelium (RPE) cell monolayer with low level of reactive oxygen species (ROS) is important for regenerative RPE‐based therapies. However, it is still challenging to culture RPE monolayer with low ROS level on soft substratesin vitro. To address this, we developed cytocompatible hydrogels to culture human RPE cell monolayer for future use in regenerative RPE‐based therapies. The cell adhesion, proliferation, monolayer formation, morphology, survival, and ROS level of human ARPE‐19 cells cultured on the surfaces of negatively charged poly (2‐acrylamido‐2‐methyl propane sulfonic sodium) (PNaAMPS) and neutral poly(N,N‐dimethylacrylamide) (PDMAAm) hydrogels with different stiffness were investigated. The importance of hydrogel stiffness on the cell function was firstly highlighted on the base of determined optimal Young's modulus for cultivation of RPE cell monolayer with relatively low ROS level. The construction of RPE cell monolayer with low ROS level on the PNaAMPS hydrogel may hold great potential as promising candidates for transplantation of RPE cell monolayer‐hydrogel construct into the subretinal space to repair retinal functions. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2258–2267, 2014.

Từ khóa


Tài liệu tham khảo

10.1016/j.ophtha.2011.05.026

10.1093/hmg/ddq341

10.1016/j.biomaterials.2008.10.045

10.1111/j.1751-1097.2012.01228.x

Stramm LE, 1989, Retinal pigment epithelial glycosaminoglycan metabolism: Intracellular versus extracellular pathways, In vitro studies in normal and diseased cells. Invest Ophthalmol Vis Sci, 30, 2118

DingJD JohnsonLV HerrmannR FarsiuS SmithSG GroelleM MaceBE SullivanP JamisonJA KellyU et al.Anti‐amyloid therapy protects against retinal pigmented epithelium damage and vision loss in a model of age‐related macular degeneration.Proc Natl Acad Sci USA2011;108:E279–E287.

10.1136/bjo.2003.039131

10.1021/ja052773z

10.1016/S0142-9612(01)00171-5

10.1016/j.biomaterials.2012.02.040

10.1016/S0142-9612(01)00172-7

10.2217/nnm.10.12

10.1016/j.actbio.2011.01.011

10.1016/j.biomaterials.2006.11.023

10.1016/j.jconrel.2012.06.039

Lai JY, 2010, J Biomater Sci Polym Ed, 277

10.1021/mz200121k

10.1016/j.biomaterials.2011.01.058

10.1016/S0014-4835(03)00023-X

10.1111/j.1751-1097.2008.00506.x

10.1002/jcp.20439

10.1038/nnano.2006.91

10.1016/j.biomaterials.2005.03.018

10.1002/jbm.a.32287

10.1039/b818586g

10.1002/jbm.a.31869

10.1016/j.biomaterials.2004.11.025

10.1016/j.biomaterials.2006.12.005

10.1007/s11431-012-4857-y

10.1089/ten.tea.2009.0647

10.1016/j.actbio.2009.07.033

10.1089/ten.tea.2011.0034

10.1167/iovs.10-6374

Roehlecke C, 2009, The influence of sublethal blue light exposure on human RPE cells, Mol Vis, 15, 1929

10.1002/adma.200304907

10.1016/j.biomaterials.2010.11.065

10.1016/j.jnutbio.2009.09.006

10.1016/j.jnutbio.2009.10.010

10.1016/j.jnutbio.2011.05.006

10.1021/ma9511917

10.1007/BF01007836

10.1002/jbm.a.30417

Treharne AJ, 2012, Developing methacrylate‐based copolymers as an artificial Bruch's membrane substitute, J Biomed Mater Res A, 100, 2358, 10.1002/jbm.a.34178

10.1002/jcp.21424

10.1016/j.exer.2009.05.011

Wankun X, 2011, Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro, Mol Vis, 17, 3512

ChenYM LiuJK FengZH LiuZQ.Preparation of hydrogel scaffolds for promoting retinal pigment epithelium cells proliferation. Chinese Patent 2011.201110191554.0.

10.1089/ten.tea.2011.0097

10.1002/adfm.200800566

10.1016/j.ab.2007.04.004

10.1002/jbm.a.31043

Dong K, 2012, Self‐healing gels: Structure, performance and future perspective, Sci China: Chem, 42, 741

10.1039/c2py20627g

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 102 Số 7

2258-2267

Thông tin tác giả