Acquisition of Invasive Phenotype in Gallbladder Cancer Cells via Mutual Interaction of Stromal Fibroblasts and Cancer Cells as Mediated by Hepatocyte Growth Factor

Wiley - Tập 87 Số 7 - Trang 702-710 - 1996
Kunio Matsumoto1, Kazuhiko Date2, Hideo Shimura3, Toshikazu Nakamura2
1Division of Biochemistry, Osaka University Medical School.
2Division of Biochemistry, Biomedical Research Center, Osaka University Medical School, 2‐2 Yamadaoka, Suita, Osaka 565
3First Department of Surgery, Kyushu University Faculty of Medicine, 3‐1‐1 Maidashi, Higashi‐ku, Fukuoka 812‐82

Tóm tắt

Growth and motility of carcinoma cells are regulated through their interactions with host stromal cells, i. e., tumor‐stromal interactions. Hepatocyte growth factor (HGF), a ligand for c‐Met tyrosine kinase, is a stromal‐derived regulator of growth, motility, and morphogenesis. HGF stimulated proliferation and motility of GB‐d1 gallbladder carcinoma cells from a patient with gallbladder cancer. HGF induced in vitro invasion of GB‐d1 cells into a collagen gel matrix, and this potent, invasive effect was not seen with epidermal growth factor, transforming growth factor‐β1, basic fibroblast growth factor, or platelet‐derived growth factor. Although GB‐d1 did not produce HGF, the cells did produce a factor which enhances HGF production in human skin fibroblasts, and this factor proved to be interleuldn‐1β (IL‐1β). When GB‐d1 cells were co‐cultured with fibroblasts such that a collagen gel matrix was layered between the GB‐d1 cells and fibroblasts, GB‐d1 cells invaded the gel, but invasion of the cells in the co‐culture system was inhibited by antibodies against HGF and partially inhibited by antibodies against IL‐1β. Thus, GB‐d1 cell‐derived IL‐1β stimulates HGF production in stromal fibroblasts and HGF up‐regulated in the fibroblasts induces invasion of GB‐d1 cells. The looped interaction of carcinoma cells and stromal fibroblasts mediated by HGF and a HGF‐inducer such as IL‐1β may be one mechanism which would explain the acquisition of malignant phenotype through tumor‐stromal interactions.

Từ khóa


Tài liệu tham khảo

10.1016/S0065-230X(08)60725-3

Saxen L., 1976, “The Cell Surface in Animal Embryogenesis and Development, 331

10.1016/0045-6039(85)90481-6

10.1016/S0074-7696(08)61219-X

Matsumoto K., 1993, “Hepatocyte Growth Factor‐Scatter Factor (HGF‐SF) and the C‐Met Receptor, 225

10.1073/pnas.87.1.75

Picard O., 1986, Fibroblast‐dependent tumorgenicity of cells in nude mice: implication for implantation of metastases, Cancer Res, 46, 3290

10.1111/j.1600-0714.1989.tb01350.x

10.1002/bjs.1800801104

10.1083/jcb.129.5.1177

10.1016/0092-8674(91)90363-4

10.1002/hep.1840170618

Soriano J. V., 1995, Hepatocyte growth factor stimulates extensive development of branching duct‐like structures by cloned mammary gland epithelial cells, J. Cell Sci, 108, 413, 10.1242/jcs.108.2.413

10.1073/pnas.92.10.4412

10.1016/0006-291X(91)91924-2

10.1083/jcb.119.3.629

10.1073/pnas.90.5.1937

10.1073/pnas.86.15.5844

10.1073/pnas.88.16.7001

10.1002/j.1460-2075.1991.tb07836.x

Furlong R. A., 1991, Comparison of biological and immunochemical properties indicates that scatter factor and hepatocyte growth factor are indistinguishable, J. Cell Sci, 100, 173, 10.1242/jcs.100.1.173

10.1083/jcb.123.1.223

10.1016/0014-5793(87)80475-1

10.1038/342440a0

10.1126/science.1846706

Naldini L., 1991, Hepatocyte growth factor stimulates the tyrosine kinase activity of the receptor encoded by the proto‐oncogene c‐MET, Oncogene, 6, 501

10.1073/pnas.90.13.6140

Ronsin C., 1993, A novel putative receptor protein kinase of the met family, Oncogene, 8, 1195

10.1111/j.1349-7006.1995.tb02450.x

10.1016/S0006-291X(05)80212-8

Okada F., 1989, Purification and structural analysis of a latent form of transforming growth factor‐β from rat platelets, J. Biochem, 106, 304, 10.1093/oxfordjournals.jbchem.a122849

Matsumoto K., 1992, Negative regulation of hepatocyte growth factor gene expression in human lung fibroblasts and leukemic cells by transforming growth factor‐β1 and glucocorticoids, J. Biol. Chem, 267, 24917, 10.1016/S0021-9258(19)73982-9

10.1016/0006-291X(92)92375-8

Matsumoto K., 1994, Hepatocyte growth factor/scatter factor induces tyrosine phosphorylation of focal adhesion kinase (p125FAK) and promotes migration and invasion of oral squamous cell carcinoma cells, J. Biol. Chem, 269, 31807, 10.1016/S0021-9258(18)31767-8

10.1083/jcb.111.5.2097

Takaishi K., 1994, Involvement of rho p21 small GTP‐binding protein and its regulatory protein in the HGF‐induced cell motility, Oncogene, 9, 273

10.1128/MCB.15.2.1110

10.1247/csf.18.117

10.3109/15419069409097261

Pepper M. S., 1992, Hepatocyte growth factor increases urokinase‐type plasminogen activator (u‐PA) and u‐PA receptor expression in Mardin‐Darby canine kidney epithelial cells, J. Biol. Chem, 267, 20493, 10.1016/S0021-9258(19)88729-X

10.1093/oxfordjournals.jbchem.a124958

10.1128/MCB.12.11.5152

Kanda H., 1993, Hepatocyte growth factor transforms immortalized liver epithelial cells, Oncogene, 8, 3047

Bellusci S., 1994, Creation of an HGF/SF autocrine loop in carcinoma cells induces invasive properties associated with increased tumorigenicity, Oncogene, 9, 1091

10.1016/0304-3835(94)90325-5

Lui C., 1992, Overexpression of c‐met proto‐oncogene but not epidermal growth factor receptor or c‐erbB‐2 in primary human colorectal carcinomas, Oncogene, 7, 181

Renzo M. F., 1992, Overexpression of the c‐met/HGF receptor gene in human thyroid carcinomas, Oncogene, 7, 2549

10.1016/0006-291X(92)91548-5

Rygaard K., 1993, Growth inhibition and change in morphology and motility of SCLC cell lines by hepatocyte growth factor/scatter factor, Int. J. Oncol, 2, 991

10.1007/BF00121166

10.1089/dna.1994.13.1189

Seslar S. P., 1993, Regulation of fibroblast hepatocyte growth factor/scatter factor expression by human breast carcinoma cell lines and peptide growth factors, Cancer Res, 53, 1233

10.1083/jcb.127.1.225

Yamashita J., 1994, Immunoreactive hepatocyte growth factor is a strong and independent predictor of recurrence and survival in human breast cancer, Cancer Res, 54, 1630

Yamashita J., 1993, Immunoreactive hepatocyte growth factor is present in tissue extracts from human breast cancer but not in conditioned medium of human breast cancer cell lines, Res. Commun. Chem. Pathol Pharmacol, 82, 249

Thông tin
Thông tin xuất bản

Wiley

Tập 87 Số 7

702-710

Thông tin tác giả