Acetylcholinesterase‐inhibitor hydrolysates obtained from ‘in vitro’ enzymatic hydrolysis of mannoproteins extracted from different strains of yeasts

International Journal of Food Science and Technology - Tập 51 Số 2 - Trang 300-308 - 2016
Pablo Gabriel Spontón1,2, Roque Spinelli2, Silvina R. Drago3, Georgina Tonarelli2, Arturo Carlos Simonetta1
1Cátedras de Microbiología y Biotecnología Dto. de Ingeniería en Alimentos FIQ‐UNL Stgo. del Estero 2829, 3000 Santa Fe Argentina
2Laboratorio de Péptidos Bioactivos Dto. de Química Orgánica FBCB‐UNL Pje. El Pozo, 3000 Santa Fe Argentina
3Instituto de Tecnología en Alimentos FIQ‐UNL Stgo. del Estero 2829, 3000 Santa Fe Argentina

Tóm tắt

SummaryIn vitro inhibitory activity against acetylcholinesterase (AChE) of peptides obtained by enzymatic hydrolysis of mannoproteins extracted from strains of yeasts was investigated. Yeast mannoproteins were extracted from strains belonging to the genera Brettanomyces, Candida, Pichia and Saccharomyces isolated from dairy products. They were obtained by heat treatment in citrate buffer and purified by affinity chromatography with concanavalin A. Each purified extract was subsequently hydrolysed with proteolytic enzymes (trypsin, pepsin, chymotrypsin and proteinase K) applied individually or in combination, thus generating smaller peptides. Inhibitory activity of the latter against AChE was determined. The molecular weight of mannoproteins, determined by SDS‐PAGE, was between 6.5 and 30 kDa. As regards AChE inhibition, a preliminary screening of all hydrolysed extracts was performed, yielding variable results with 59% maximum inhibition. Subsequently, when inhibitory concentration 50 (IC50) was determined, the extracts showed higher inhibitory activity (between 6.75 and 12.3 mg mL−1). Results showed that the mannoproteins separated from yeast strains of food origin generated bioactive peptides by enzymatic hydrolysis, which can be of interest to the manufacturing of food with potential functional properties.

Từ khóa


Tài liệu tham khảo

10.1201/9780203507438.ch7

10.1111/j.1365-2621.2009.02166.x

10.1002/elps.1150080203

Cameron D., 1988, The mannoprotein of Saccharomyces cerevisiae is an effective bioemulsifier, Applied and Environmental Microbiology, 54, 1420, 10.1128/aem.54.6.1420-1425.1988

10.1016/j.foodchem.2011.08.047

Devomoy K.L., 2003, Current drug targets Alzheimer's disease treatment, Drug Development Research, 56, 267

10.1021/jf9905020

10.1021/jf0002443

10.1016/0006-2952(61)90145-9

Filho J., 2006, Natural products inhibitors of the enzyme acetylcholinesterase, Brazilian Journal of Pharmacognosy, 16, 258, 10.1590/S0102-695X2006000200021

Freeze H.H., 2001, Special considerations for glycoproteins and their purification, Current Protocols in Molecular Biology, 17, 17.1

Fujimoto E.K., 1985, Measurement of protein using bicinchoninic acid, Analytical Biochemistry, 150, 76, 10.1016/0003-2697(85)90442-7

Gañan M. Martínez‐Rodríguez A.J.&Carrascosa A.V.(2007).Manoproteínas de levadura y su aplicación en la industria cárnica. Dpto. Microbiología Instituto de Fermentaciones Industriales Consejo Superior de Investigaciones Científicas C/Juan de la Cierva 3 28006 Madrid.www.ifi.csic.es. No. 55.

10.4315/0362-028X-72.1.55

10.1016/j.phrs.2003.11.017

10.1021/jf0202741

10.2174/138527206776894410

10.1128/IAI.70.10.5485-5493.2002

10.1016/S0965-1748(99)00088-0

10.2174/1381612033454892

Kraut D., 2000, Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride, Molecular Pharmacology, 57, 1243

10.1038/227680a0

10.1016/S0024-3205(02)02034-9

10.1016/S0958-6946(98)00059-4

10.2174/1570159X11311040004

10.1111/j.1365-2621.2001.tb04614.x

10.1021/jf0615496

10.31482/mmsl.2012.005

10.1016/j.phymed.2007.02.002

Singh A.P., 2003, The role of natural products in pharmacotherapy of Alzheimer disease, Ethnobotanical Leaflets, 7, 1

So L., 1968, The interaction of concanavalin A with R‐mannans from a variety of microorganisms, Journal of Biological Chemistry, 243, 2003

10.1002/ddr.10087

10.1016/j.jep.2006.06.014

10.1111/j.1541-4337.2005.tb00075.x

Zarotsky V., 2003, Galantamine hydrobromide: an agent for Alzheimer's disease, American Journal of Health‐System Pharmacy, 60, 446, 10.1093/ajhp/60.5.446

Thông tin
Thông tin xuất bản

International Journal of Food Science and Technology

Tập 51 Số 2

300-308

Thông tin tác giả