Acclimation of metabolism to light in <scp><i>A</i></scp><i>rabidopsis thaliana</i>: the glucose 6‐phosphate/phosphate translocator <scp>GPT</scp>2 directs metabolic acclimation

Plant, Cell and Environment - Tập 38 Số 7 - Trang 1404-1417 - 2015
Beth C. Dyson1, J. William Allwood2, Regina Feil3, Yun Xu2, Matt Miller1, Caroline Bowsher1, Royston Goodacre2, John E. Lunn3, Giles N. Johnson1
1Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
2School of Chemistry and Manchester Institute of Biotechnology University of Manchester Manchester M1 7DN UK
3Max-Planck-Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany

Tóm tắt

AbstractMature leaves of plants transferred from low to high light typically increase their photosynthetic capacity. In Arabidopsis thaliana, this dynamic acclimation requires expression of GPT2, a glucose 6‐phosphate/phosphate translocator. Here, we examine the impact of GPT2 on leaf metabolism and photosynthesis. Plants of wild type and of a GPT2 knockout (gpt2.2) grown under low light achieved the same photosynthetic rate despite having different metabolic and transcriptomic strategies. Immediately upon transfer to high light, gpt2.2 plants showed a higher rate of photosynthesis than wild‐type plants (35%); however, over subsequent days, wild‐type plants acclimated photosynthetic capacity, increasing the photosynthesis rate by 100% after 7 d. Wild‐type plants accumulated more starch than gpt2.2 plants throughout acclimation. We suggest that GPT2 activity results in the net import of glucose 6‐phosphate from cytosol to chloroplast, increasing starch synthesis. There was clear acclimation of metabolism, with short‐term changes typically being reversed as plants acclimated. Distinct responses to light were observed in wild‐type and gpt2.2 leaves. Significantly higher levels of sugar phosphates were observed in gpt2.2. We suggest that GPT2 alters the distribution of metabolites between compartments and that this plays an essential role in allowing the cell to interpret environmental signals.

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Plant, Cell and Environment

Tập 38 Số 7

1404-1417

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