Abundant nuclear copies of mitochondrial origin (NUMTs) in the Aedes aegypti genome

Insect Molecular Biology - Tập 18 Số 6 - Trang 705-713 - 2009
William C. Black1, Scott A. Bernhardt1
1Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA

Tóm tắt

AbstractA portion of the Aedes aegypti mitochondrial NADH dehydrogenase subunit 4 gene (ND4) was amplified using PCR with a 42 °C annealing temperature. Amplified fragments from individual mosquitoes were similar to ND4 but contained multiple segregating sites. We suspected that nuclear copies of mitochondrial origin (NUMTs) exist in the Ae. aegypti genome. A BlastN search in VectorBase with the entire Ae. aegypti mitochondrial genome identified 233 NUMTs comprising 110 178 bp in 145 supercontigs. At a density of 0.080 bp/kb, this represents the second highest density of NUMTs in an insect genome and the highest in Diptera. Analyses of flanking sequences suggested that Ae. aegypti NUMTs arise through mtDNA leakage from damaged mitochondria followed by breakage and nonhomologous recombination, rather than through duplicative processes such as transposition or molecular drive.

Từ khóa


Tài liệu tham khảo

10.1093/molbev/msm068

10.1093/oxfordjournals.molbev.a026320

10.1016/S0169-5347(01)02151-6

10.1007/s00239-003-2485-7

10.1007/978-94-009-1535-0_31

10.4269/ajtmh.2005.72.434

10.1007/s10764-008-9303-4

10.4269/ajtmh.2000.62.200

10.4269/ajtmh.2002.66.213

10.1073/pnas.83.22.8813

10.1093/molbev/msl149

10.1016/S0168-9525(01)02312-5

10.1186/1471-2148-7-67

10.1186/1471-2164-9-267

Lopez J.V., 1994, Numt, a recent transfer and tandem amplification of mitochondrial‐DNA to the nuclear genome of the domestic cat, J Mol Evol, 39, 174, 10.1007/BF00163806

10.1534/genetics.107.079624

10.1371/journal.pntd.0000468

10.1111/j.1365-2583.2007.00771.x

10.1093/oxfordjournals.molbev.a003971

10.1126/science.1138878

Paduan K.D., 2008, Mitochondrial DNA polymorphism and heteroplasmy in populations of Aedes aegypti in Brazil, J Med Entomol, 45, 59, 10.1093/jmedent/45.1.59

10.1093/molbev/msm055

10.1186/1471-2148-4-17

10.1016/S1673-8527(08)60066-1

10.1038/47076

10.1093/molbev/msh110

10.1093/oxfordjournals.molbev.a025612

10.1038/sj.hdy.6800484

10.1007/s10709-007-9145-6

Urdaneta‐Marquez L., 2008, Genetic relationships among Aedes aegypti collections in Venezuela as determined by mitochondrial DNA variation and nuclear single nucleotide polymorphisms, Am J Trop Med Hyg, 78, 479, 10.4269/ajtmh.2008.78.479

10.1017/S0016672300029979

10.1101/gr.227202. Article published online before print in May 2002

10.1046/j.1365-294X.1996.00078.x

Thông tin
Thông tin xuất bản

Insect Molecular Biology

Tập 18 Số 6

705-713

Thông tin tác giả