Sự phong phú và đa hình của các trình tự DNA lặp đơn giản trong Brassica napus L.

Theoretical and Applied Genetics - Tập 85 - Trang 994-1000 - 1993
G. B. Poulsen1, G. Kahl2, K. Weising2
1Biotechnology Group, Danish Research Service for Plant and Soil Science, Lyngby, Denmark
2Plant Molecular Biology Group, University of Frankfurt, Frankfurt am Main, FRG

Tóm tắt

Gen của Brassica napus đã được nghiên cứu thông qua việc phân tích dấu vân tay DNA với sáu mồi oligonucleotide tổng hợp bổ sung cho các trình tự lặp đơn giản, cụ thể là (GATA)4, (GACA)4, (GGAT)4, (CA)8, (CT)8 và (GTG)5. Trong khi tất cả các mô hình trình tự đều có mặt trong gen B. napus, cách tổ chức và sự phong phú của chúng khác nhau đáng kể. Trong số các mồi đã được khảo sát, (GATA)4 cho thấy mức độ đa hình nội loài cao nhất và phân biệt không chỉ giữa các giống mà ngay cả giữa các cá thể khác nhau thuộc cùng một giống. Ngược lại, (GTG)5, (GACA)4 và (GGAT)4 tạo ra các mẫu dấu vân tay tương đối đồng nhất trên các giống khác nhau, trong khi sự lai ghép với (CT)8 và (CA)8 chỉ cho ra một vài băng yếu chồng lên một vết mờ. Cặp isoschizomeric Hpa II và Msp I cho thấy sự hiện diện của các cytosin methyl hóa gần các trình tự (GATA)m lặp lại. Tính khả thi của sự đa hình của các trình tự lặp đơn giản như là các dấu phân tử cho các loài Brassica được thảo luận.

Từ khóa

#Brassica napus #DNA #dấu vân tay #đa hình #trình tự lặp đơn giản

Tài liệu tham khảo

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