Ablation of eosinophils with anti‐IL‐5 antibody enhances the survival of intracranial worms of Angiostrongylus cantonensis in the mouse

Parasite Immunology - Tập 15 Số 6 - Trang 349-354 - 1993
Osamu Sasaki1, Hiroko Sugaya2, Kazuto Ishida2, Kentaro Yoshimura2
1Laboratory of Animal Hygiene, Department of Animal Science, Akita Prefectural College of Agriculture, Ogata, Akita 010‐04, Japan
2Department of Parasitology, Akita University School of Medicine, Hondo, Akita 010, Japan

Tóm tắt

SummaryEffects of depressed eosinophilia on the development of Angiostrongylus cantonensis in the mouse were studied using monoclonal rat anti‐mouse‐interleukin‐5 antibody (anti‐IL‐5). The administration of anti‐IL‐5 strongly depressed peripheral, cerebrospinal fluid (CSF) and medullary eosinophilic responses in mice infected with A. cantonensis, when compared with groups treated with phosphate‐buffered saline solution (PBS) alone or isotype‐matched rat IgG. There was no significant difference in A. cantonensis antigen specific IgG and IgE antibody responses between rat IgG treated and anti‐IL‐5 treated mice. Intracranial worm recovery in anti‐IL‐5 treated mice was consistently high throughout the course of the study and some worms migrated from the brain to the lungs. By contrast, almost all the intracranial worms in the mouse groups treated with PBS alone or rat IgG died before day 32. These data clearly indicate that IL‐5 is essential for eosinophil responses in A. cantonensis infected mice and also that eosinophils serve as a potential effector cell in the killing of the intracranial worms in mice.

Từ khóa


Tài liệu tham khảo

Alicata J. E., 1970, Angiostrongylosis in the Pacific and Southeast Asia

Bond M. W., 1987, A mouse T cell product that preferentially enhances IgA production. II. Physicochemical characterization, Journal of Immunology, 139, 3691, 10.4049/jimmunol.139.11.3691

10.1016/S0065-308X(08)60287-0

10.1126/science.2787531

Coffman R. L., 1987, A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization, Journal of Immunology, 139, 3685, 10.4049/jimmunol.139.11.3685

10.1016/0035-9203(87)90267-7

Korenaga M., 1991, The role of interleukin‐5 in protective immunity to Strongyloides venezuelensis infection in mice, Immunology, 72, 502

10.1016/S0021-9258(19)52451-6

10.1146/annurev.iy.07.040189.001045

10.1016/0014-4894(89)90125-2

Rennick D. M., 1990, In vivo administration of antibody to interleukin‐5 inhibits increased generation of eosinophils and their progenitors in bone marrow of parasitized mice, Blood, 76, 312, 10.1182/blood.V76.2.312.bloodjournal762312

Schimpl A., 1975, A third signal in B cell activation given by TRF, Transplantation Review, 23, 176

Sher A., 1990, Ablation of eosinophil and IgE responses with anti‐IL‐5 or anti‐IL‐4 antibodies fails to affect immunity against Schistosoma mansoni in the mouse, Journal of Immunology, 145, 3911, 10.4049/jimmunol.145.11.3911

10.1073/pnas.87.1.61

10.1111/j.1365-3024.1988.tb00209.x

10.1084/jem.156.6.1821

Swain S. L., 1988, Characterization of T helper 1 and 2 cell subsets in normal mice. Helper T cells responsible for IL‐4 and IL‐5 production are present as precursors that require priming before they develop into lymphokine‐secreting cells, Journal of Immunology, 141, 3445, 10.4049/jimmunol.141.10.3445

Yamaguchi Y., 1990, In vivo changes of hemopoietic progenitors and the expression of interleukin 5 gene in eosinophilic mice infected with Toxocara canis, Experimental Hematology, 18, 1152

Yoshimura K., 1989, Current Concepts in Parasitology, 7

10.1016/0020-7519(79)90097-3

10.1007/BF00928117

Yoshimura K., 1976, Angiostrongylus cantonensis: Lymphoid cell responsiveness and antibody production in rats, American Journal of Tropical Medicine and Hygiene, 25, 99, 10.4269/ajtmh.1976.25.99

10.1111/j.1365-3024.1988.tb00231.x

Yoshimura K., 1983, Antibody‐dependent adherence and damage to the young adult worms of Angiostrongylus cantonensis by rat eosinophils in vitro, Japanese Journal of Parasitology, 32, 489

Thông tin
Thông tin xuất bản

Parasite Immunology

Tập 15 Số 6

349-354

Thông tin tác giả