APC/C-Cdh1-mediated degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus

Genes and Development - Tập 22 Số 1 - Trang 79-90 - 2008
Clara Visintin1, Brett N. Tomson2, Rami Rahal2, Jennifer L. Snead3, Michael S. Cohen3, Jack Taunton3, Angelika Amon2, Rosella Visintin4
1Department of Experimental Oncology, European Institute of Oncology, Milano 20141, Italy
2Massachusetts Institute of Technology,
3University of California at San Francisco
4IRCCS Istituto Europeo di Oncologia - Milano

Tóm tắt

In the budding yeastSaccharomyces cerevisiae, the protein phosphatase Cdc14 triggers exit from mitosis by promoting the inactivation of cyclin-dependent kinases (CDKs). Cdc14’s activity is controlled by Cfi1/Net1, which holds and inhibits the phosphatase in the nucleolus from G1 until metaphase. During anaphase, two regulatory networks, the Cdc14 Early Anaphase Release (FEAR) network and the Mitotic Exit Network (MEN), promote the dissociation of Cdc14 from its inhibitor, allowing the phosphatase to reach its targets throughout the cell. The molecular circuits that trigger the return of Cdc14 into the nucleolus after the completion of exit from mitosis are not known. Here we show that activation of a ubiquitin ligase known as the Anaphase-Promoting Complex or Cyclosome (APC/C) bound to the specificity factor Cdh1 triggers the degradation of the Polo kinase Cdc5, a key factor in releasing Cdc14 from its inhibitor in the nucleolus.

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Tài liệu tham khảo

10.1016/0092-8674(94)90443-X

10.1126/science.1099402

10.1126/science.3018930

10.1038/35099020

10.1016/S0962-8924(01)01928-6

10.1016/S0960-9822(98)70201-5

10.1101/gad.10.24.3081

10.1074/jbc.C300059200

10.1016/0378-1119(88)90185-0

10.1093/emboj/19.23.6475

10.1101/gad.1013102

Hu,, 2002, Bub2 is a cell cycle regulated phospho-protein controlled by multiple checkpoints, Cell Cycle, 1, 351, 10.4161/cc.1.5.154

10.1016/S0092-8674(01)00580-3

10.1091/mbc.9.10.2803

Johnson,, 1992, Ubiquitin as a degradation signal, EMBO J., 11, 497, 10.1002/j.1460-2075.1992.tb05080.x

10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U

10.1146/annurev.genet.35.102401.091334

Nasmyth,, 2001, Splitting the chromosome: Cutting the ties that bind sister chromatids, Novartis Found. Symp., 237, 113, 10.1002/0470846666.ch10

10.1093/emboj/20.22.6359

10.1083/jcb.200112085

10.1016/S1097-2765(02)00540-3

10.1016/j.cell.2006.03.038

10.1016/S1534-5807(04)00128-5

10.1016/S0092-8674(00)80529-2

Shah,, 2001, The Spo12 protein of Saccharomyces cerevisiae: A regulator of mitotic exit whose cell cycle-dependent degradation is mediated by the anaphase-promoting complex, Genetics, 159, 965, 10.1093/genetics/159.3.965

10.1093/emboj/17.5.1336

10.1038/46080

10.1016/S0092-8674(00)80733-3

10.1146/annurev.genet.38.072902.093051

10.1016/S0092-8674(02)00618-9

10.1016/j.cub.2004.03.009

10.1038/ncb940

Visintin,, 2001, Regulation of the mitotic exit protein kinases Cdc15 and Dbf2, Mol. Biol. Cell, 12, 2961, 10.1091/mbc.12.10.2961

10.1016/S1097-2765(00)80286-5

10.1038/19775

10.1091/mbc.E03-02-0095

10.1016/S0092-8674(03)00121-1

10.1038/nature00856

10.1016/S0006-291X(02)00544-2

10.1126/science.282.5394.1721

10.1038/nmeth764

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Genes and Development

Tập 22 Số 1

79-90

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