ALKBH9C, a potential RNA m6A demethylase, regulates the response of Arabidopsis to abiotic stresses and abscisic acid

Plant, Cell and Environment - Tập 45 Số 12 - Trang 3566-3581 - 2022
Umme Amara1, Yasira Shoaib1, Hunseung Kang1
1Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea

Tóm tắt

AbstractAlthough several studies have shown that AlkB homolog (ALKBH) proteins are potential RNA demethylases (referred to as ‘erasers’), biological functions of only a few ALKBH proteins have been characterized to date. In this study, we determined the function of ALKBH9C (At4g36090) in seed germination and seedling growth of Arabidopsis thaliana in response to abiotic stress and abscisic acid (ABA). Seed germination of the alkbh9c mutant was delayed in response to salt, drought, cold and ABA. Moreover, seedling growth of the mutant was repressed under salt stress or ABA but enhanced under drought conditions. Notably, the stress‐responsive phenotypes were associated with the altered expression of several m6A‐modified transcripts related to salt, drought or ABA response. Global m6A levels were increased in the alkbh9c mutant, and ALKBH9C bound to m6A‐modified RNAs and had in vitro m6A demethylase activity, suggesting its potential role as an m6A eraser. The m6A levels in several stress‐responsive genes were increased in the alkbh9c mutant, and the stability of m6A‐modified transcripts was altered in the mutant. Collectively, our results suggest that m6A eraser ALKBH9C is crucial for seed germination and seedling growth of Arabidopsis in response to abiotic stresses or ABA via affecting the stability of stress‐responsive transcripts.

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Plant, Cell and Environment

Tập 45 Số 12

3566-3581

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