A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide
Tóm tắt
Excitable cells in many endocrine and neuronal systems display rhythms with periodicities on the order of many minutes. To observe firing patterns that represent the output of these rhythms requires a recording technique that can monitor electrophysiological activity for several hours without affecting cell behavior. A targeted extracellular approach (also known as loose-patch) accomplishes this objective. Because low resistance seals (<20 MΩ) do not influence the cell membrane and because the normal intracellular milieu is maintained, this approach is the least invasive method for monitoring the endogenous electrical activity of single cells. In this report, we detail our use of this technique to record the firing patterns of gonadotropin-releasing hormone (GnRH) neurons in brain slices continuously for several hours.
Tài liệu tham khảo
Roberts WM, Almers W. Patch voltage clamping with low-resistance seals: loose patch clamp.Methods Enzymol 1992;207:155–176
Stuhmer W, Roberts WM, Almers W. The loose patch clamp. In Sakmann B, Neher (eds): “Single-Channel Recording.” 1983 2nd ed. New York: Plenum Press, pp. 123–132.
Anson BD, Roberts WM. A novel voltage clamp technique for mapping ionic currents form cultured skeletal myotubes.Biophysical Journal 1998; 74:2963–2972.
Clarke IJ, Cummins JT. The temporal relationship between gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretion in ovariectomized ewes.Endocrinology 1982; 111:1737–1739.
Levine JE, Ramirez VD. Luteinizing hormone-releasing hormone release during the rat estrous cycle and after ovariectomy, as estimated with push-pull cannulae.Endocrinology 1982; 111:1439–1448.
Moenter SM, Caraty A, Locatelli A, Karsch FJ. Pattern of gonadotropin-releasing hormone (GnRH) secretion leading up to ovulation in the ewe: existence of a preovulatory GnRH surge.Endocrinology 1991; 129:1175–1182.
Nunemaker CS, DeFazio RA, Moenter SM. Estradiol-sensitive afferents modulate long-term episodic firing patterns of GnRH neurons.Endocrinology 2002; 143:2284–2292.
Hille B. Ion channels of excitable membranes. 3rd ed. Sunderland, MA: Sinauer Associates, Inc.; 2001.
Sherman-Gold R (ed). The axon guide for electrophysiology and biophysics laboratory techniques. Axon Instruments, 1993. No longer available in print, but is available on-line at http://www.axon.com.
Suter KJ, Song WJ, Sampson TL, Wuarin JP, Saunders JT, Dudek FE, Moenter SM. Genetic targeting of green fluorescent protein to gonadotropin-releasing hormone neurons: characterization of whole-cell electrophysiological properties and morphology.Endocrinology 2000; 141:412–419.
Liu HS, Jan MS, Chou CK, Chen PH, Ke NJ. Is green fluorescent protein toxic to the living cells?Biochem Biophys Res Comm 1999; 260:712–717.
Velhuis JD, Johnson ML. Cluster analysis: a simple versatile, and robust algorithm for endocrine pulse detection.Am J Physiol Endocrinol Metab 1986; 250:E486-E493.
Nunemaker CS, DeFazio RA, Geusz ME, Herzog ED, Pitts GR, Moenter SM. Long-term recordings of networks of immortalized GnRH neurons reveal episodic patterns of electrical activity.J Neurophysiol 2001; 86:86–93.
Nunemaker CS, Straume M, DeFazio RA, Moenter SM. Gonadotropin-releasing hormone neurons generate interacting rhythms in multiple time domains.Endocrinology March 2003;144 (in press).
Milton RL, Caldwell JH. How do patch clamp seals form? A lipid bleb model.Pflugers Arch Eur J Phys 1990; 416:758–762.
Reuss S, Vollrath L. Electrophysiological properties of rat pinealocytes: evidence for circadian and ultradian rhythms.Exp Brain Res 1984; 55:455–461.
Schenda J, Vollrath L. Single-cell recordings from chick pineal glands in vitro reveal ultradian and circadian oscillations.Cell Mol Life Sci 2000; 57:1785–1792.