James W. Allen1, Charles F. Shuler1, Robert W. Mendes2, Samuel A. Latt1
1Department of Pediatrics, Mental Health Retardation Center, Childrens Hospital Medical Center, Harvard Medical School, and
2Department of Industrial Pharmacy, Massachusetts College of Pharmacy, Boston, Mass.
Tóm tắt
A small tablet of 5-bromodeoxyuridine (BrdU), implanted subcutaneously in a mouse, provides sustained release of base analog sufficient to effect substitution of DNA throughout an entire replication period. As illustrated by studies of mouse bone-marrow and spleen cells in the presence or absence of cyclophosphamide, this depot method of BrdU administration greatly simplifies in vivo analysis of sister-chromatid-exchange formation.
