A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells

Genes and Development - Tập 21 Số 24 - Trang 3238-3243 - 2007
Ingrid Ibarra1,2, Yaniv Erlich2, Senthil K. Muthuswamy2, Ravi Sachidanandam2, Gregory J. Hannon2
1Program in Genetics, Stony Brook University, Stony Brook, New York 11794, USA
2Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA

Tóm tắt

microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dβ, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.

Từ khóa


Tài liệu tham khảo

10.1261/rna.7240905

10.1038/ng1253

10.1016/j.cell.2005.08.020

Bunting,, 1996, De novo expression of transfected human class 1 aldehyde dehydrogenase (ALDH) causes resistance to oxazaphosphorine anti-cancer alkylating agents in hamster V79 cell lines. Elevated class 1 ALDH activity is closely correlated with reduction in DNA interstrand cross-linking and lethality, J. Biol. Chem., 27, 11884, 10.1074/jbc.271.20.11884

10.1093/nar/gni178

10.1242/jcs.03348

10.1038/sj.leu.2404721

10.1634/stemcells.2005-0217

10.1016/S1046-2023(03)00032-X

10.1016/j.ydbio.2006.02.007

10.1371/journal.pbio.0030236

Ginestier, C. Hur, M.H. Charafe-Jauffret, E. Monville, F. Dutcher, J. Brown, M. Jacquemier, J. Viens, P. Kleer, C. Liu, S. (2007) ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell, (in press).

10.1126/science.1122689

10.1084/jem.183.4.1797

10.1093/nar/gkj112

10.1182/blood-2005-06-2284

10.1016/S1534-5807(03)00227-2

10.1101/gad.1334005

10.1016/j.cub.2007.01.050

10.1101/gr.229202. Article published online before March 2002

10.1016/S0960-9822(02)00809-6

10.1093/nar/22.12.2183

10.1093/nar/gni113

10.1101/gad.1522907

10.1038/nmeth746

10.1038/35002607

10.1038/nature04372

10.1200/JCO.2003.03.114

10.1101/gr.6593807

10.1111/j.1432-0436.1998.00201.x

Stingl,, 2006, Purification and unique properties of mammary epithelial stem cells, Nature, 439, 993, 10.1038/nature04496

10.1038/ng1969

Thông tin
Thông tin xuất bản

Genes and Development

Tập 21 Số 24

3238-3243

Thông tin tác giả