Một chu kỳ sao chép viroid được điều chỉnh: Vai trò của RNA dài hơn chiều dài đơn vị trong quá trình sao chép viroid

Springer Science and Business Media LLC - Tập 196 - Trang 421-428 - 1984
Masayuki Ishikawa1, Tetsuo Meshi1, Takeshi Ohno1, Yoshimi Okada1, Teruo Sano2, Ichiro Ueda2, Eishiro Shikata2
1Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo, Japan
2Department of Botany, Faculty of Agriculture, Hokkaido University, Sapporo, Japan

Tóm tắt

Các RNA chuỗi dương và chuỗi âm dài hơn chiều dài đơn vị đã được phát hiện trong mô lá dưa chuột bị nhiễm viroid hop stunt (HSV) thông qua phân tích lai Northern blot sử dụng các đầu dò cụ thể cho chuỗi. Để làm rõ vai trò của các RNA dài hơn chiều dài đơn vị này trong chu kỳ sao chép của viroid, chúng tôi đã tổng hợp RNA chuỗi dương và chuỗi âm của HSV được lặp lại theo tandem in vitro từ cDNA đã được nhân bản của HSV và kiểm tra khả năng gây nhiễm của chúng. RNA chuỗi dương được lặp lại hai và bốn đơn vị có khả năng gây nhiễm, nhưng RNA chuỗi dương một đơn vị và RNA chuỗi âm một, hai và bốn đơn vị thì không có khả năng gây nhiễm. Dựa trên các dữ liệu này, chúng tôi đề xuất một mô hình vòng tròn lăn đã được điều chỉnh cho quá trình sao chép viroid.

Từ khóa

#viroid #RNA #hop stunt viroid #sao chép viroid #Northern blot hybridization #khả năng gây nhiễm

Tài liệu tham khảo

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