A reverse genetic screen in Drosophila using a deletion-inducing mutagen

Genome Biology - Tập 5 - Trang 1-10 - 2004
Knud Nairz1, Peder Zipperlen2, Charles Dearolf3, Konrad Basler2, Ernst Hafen1
1Zoologisches Institut, Universität Zürich, Zurich, Switzerland
2Institut für Molekularbiologie, Universität Zürich, Zurich, Switzerland
3Massachusetts General Hospital, Boston, USA

Tóm tắt

We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.

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Genome Biology

Tập 5

1-10

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