A reference isotope dilution headspace GC/MS method for the determination of nitrite and nitrate in meat samples

International Journal of Food Science and Technology - Tập 55 Số 3 - Trang 1110-1118 - 2020
Natasha Luckovitch1, Enea Pagliano1
1National Research Council Canada, 1200 Montreal Road, Ottawa, Ontario K1A 0R6, Canada

Tóm tắt

SummaryA novel method for the determination of nitrite and nitrate in meat products is presented. The samples were ground and extracted in hot water with the presence of and internal standards. The solution was buffered with sodium bicarbonate and reacted with triethyloxonium tetrafluoroborate to convert nitrite and nitrate into EtNO2 and EtONO2. Such derivatives could be detected by headspace GC/MS in positive chemical ionisation mode with 0.05 µg g−1 and 1.0 µg g−1 LOD. The method was used for and quantitation in the 0.5‐300 and 2.5‐300 µg g−1 ranges. The method was applied for the analysis of fifteen meat products. Despite minimal sample preparation, the headspace sampling ensured a clean chromatography for over 135 analyses (throughput ten samples per hour). The proposed method offers selective GC/MS detection combined with high‐precision isotope dilution calibration, it is suitable for metrological applications and can support regulations on meat safety (European Commission, 2011).

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Tài liệu tham khảo

10.1007/s12161-016-0789-7

10.1016/j.meatsci.2016.03.009

10.1016/j.aca.2017.04.053

10.1016/j.snb.2010.06.028

10.1002/ijc.28331

10.1016/j.foodchem.2018.11.081

10.1158/0008-5472.CAN-09-3929

10.1007/s12161-019-01529-0

10.1021/ac900865d

10.1016/j.meatsci.2016.04.011

10.2116/analsci.20.1749

European Commission, 2011, Decision No 1129/2011/EC of 11 November 2011 amending Annex II to Regulation (EC) No 1333/2008 of the European Parliament and of the Council by establishing a Union list of food additives, Official Journal of the European Union, 295, 1

10.1038/bjc.2011.549

10.1016/j.meatsci.2007.05.030

10.3945/ajcn.2008.27131

10.1016/j.foodchem.2008.11.081

10.1016/j.foodcont.2019.01.016

10.1111/j.1365-2621.2012.03041.x

International Organization for Standardization(1975a).ISO 2918:1975 – Meat and meat product – determination of nitrite content (Reference method).

International Organization for Standardization(1975b).ISO 3091:1975 – Meat and meat product – determination of nitrate content (Reference method).

10.3945/ajcn.2009.28014

10.1039/c2ay26239h

10.1016/j.talanta.2016.02.036

10.1002/jsfa.7974

10.1016/j.foodchem.2015.08.017

Merino L., 2016, Time‐dependent depletion of nitrite in pork/beef and chicken meat products and its effect on nitrite intake estimation, Food Additives & Contaminants: Part A, 33, 186

Merino L., 2017, Advances in Food and Nutrition Research, 65

10.1021/jf204611k

10.1016/j.talanta.2011.07.098

10.1016/j.aca.2014.03.018

10.1016/j.aca.2015.09.020

10.1016/j.aca.2018.03.043

10.1007/s00216-019-01803-4

10.1007/s007690050165

10.1016/j.meatsci.2017.05.007

10.1093/jaoac/89.3.712

10.1016/j.meatsci.2012.03.002

10.1016/j.saa.2015.07.028

10.1093/aje/kwp280

10.1016/S0021-9673(97)01245-4

10.1016/j.niox.2016.05.001

10.1016/j.meatsci.2018.03.012

10.1016/j.meatsci.2012.07.005

10.1146/annurev-nutr-071812-161159

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Thông tin xuất bản

International Journal of Food Science and Technology

Tập 55 Số 3

1110-1118

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