A recovery model of partial cardiopulmonary bypass in the rat

Perfusion (United Kingdom) - Tập 16 Số 3 - Trang 215-220 - 2001
Olivier Fabre1, Rachid Zegdi2, André Vincentelli3, Michèle Cambillaud4, Alain Prat3, Alain Carpentier2, Jean‐Noël Fabiani2
1Department of Thoracic and Cardiovascular Surgery, Broussais Hospital, Paris, France.
2Department of Thoracic and Cardiovascular Surgery, Broussais Hospital, Paris
3Department of Cardiac Surgery, Cardiologic Hospital, Lille
4Department of Biochemistry, Broussais Hospital, Paris

Tóm tắt

This study was undertaken to develop a recovery model of cardiopulmonary bypass (CPB) in rats. Twenty male Wistar rats (475-550 g) were anaesthetized, mechanically ventilated and the femoral vessels cannulated. The extracorporeal circulation circuit comprised a roller pump, a venous reservoir and a modified Capiox 308 paediatric membrane oxygenator. Priming consisted of 20 ml of fresh homologous blood and 15 ml of colloid. Anticoagulation was achieved with heparin (500 IU/kg). Blood gas analysis, blood pressure monitoring and survival studies were performed in CPB ( n=10) and Sham ( n=10) rats. Partial CPB was always easily established and was conducted at a flow rate of 100 ml/kg/min for 90 min. Blood gas analysis and blood pressure data did not differ between the two groups. All CPB rats survived and the 3-week follow-up period remained uneventful. The rat model of CPB was easy to perform and was associated with excellent survival. This recovery model should allow us to study the pathophysiological processes underlying post-CPB multiple organ dysfunction.

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Tài liệu tham khảo

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Thông tin xuất bản

Perfusion (United Kingdom)

Tập 16 Số 3

215-220

Thông tin tác giả