A rapid technique for analysing diets of invertebrate predators by electrophoresis
Tóm tắt
A technique has been developed for the rapid determination of some species of prey consumed by mites and insects. The method detects prey enzymes within the gut of a predator by polyacrylamide gradient gel electrophoresis and subsequent staining for esterase activity. It is sufficiently sensitive to detect fruit tree red spider mite (Panonychus ulmi) esterases within the gut of a single predacious mite (Typhlodromus pyri) for at least 31 h after feeding. The method has been used to demonstrate feeding relationships among a range of insect and mite predator and prey species in the laboratory and in the field.
Từ khóa
Tài liệu tham khảo
Brooke M. M., 1946, Precipitin tests for determining the natural predators of immature mosquitoes, Journal of the National Malaria Society, 5, 45
Devonshire A. L., 1975, Studies of the carboxylesterases of Myzus persicae resistant and susceptible to organophosphorus insecticides, Proceedings of the 8th British Insecticide and Fungicide Conference, 1, 67
Frank J. H., 1967, A serological method used in the investigation of the predators of the pupal stage of the winter moth, Operophtera brumata (L.) (Hydriomenidae), Quaestiones Entomologicae, 3, 95
Hall R. R., 1953, Evaluation of insect predator‐prey relationships by precipitin test studies, Mosquito News, 13, 199
Marinkelle C. J., 1969, Host identification of arthropod bloodmeals by agar electrophoresis, Mitteilungen der Instituto Colombo-Aleman de Invesligaciones Cientificas, 3, 29
Sergeeva T. K., 1975, The use of cross Immunoelectrophoresis for improving the sensitivity of the serological reaction when studying trophic relationships in insects. (In Russian, English Summary., Zoologicheskir zhurnal, 54, 1014