A rapid technique for analysing diets of invertebrate predators by electrophoresis

Annals of Applied Biology - Tập 90 Số 1 - Trang 7-10 - 1978
R. A. Murray1, M. G. Solomon1
1East Mailing Research Station, Maidstone, Kent ME 19 6BJ

Tóm tắt

SUMMARYA technique has been developed for the rapid determination of some species of prey consumed by mites and insects. The method detects prey enzymes within the gut of a predator by polyacrylamide gradient gel electrophoresis and subsequent staining for esterase activity. It is sufficiently sensitive to detect fruit tree red spider mite (Panonychus ulmi) esterases within the gut of a single predacious mite (Typhlodromus pyri) for at least 31 h after feeding. The method has been used to demonstrate feeding relationships among a range of insect and mite predator and prey species in the laboratory and in the field.

Từ khóa


Tài liệu tham khảo

10.1017/S0007485300010737

10.1038/2061164a0

Brooke M. M., 1946, Precipitin tests for determining the natural predators of immature mosquitoes, Journal of the National Malaria Society, 5, 45

10.2307/2275

Devonshire A. L., 1975, Studies of the carboxylesterases of Myzus persicae resistant and susceptible to organophosphorus insecticides, Proceedings of the 8th British Insecticide and Fungicide Conference, 1, 67

Frank J. H., 1967, A serological method used in the investigation of the predators of the pupal stage of the winter moth, Operophtera brumata (L.) (Hydriomenidae), Quaestiones Entomologicae, 3, 95

Hall R. R., 1953, Evaluation of insect predator‐prey relationships by precipitin test studies, Mosquito News, 13, 199

10.1016/0003-2697(68)90109-7

10.1016/S0021-9673(01)81066-9

Marinkelle C. J., 1969, Host identification of arthropod bloodmeals by agar electrophoresis, Mitteilungen der Instituto Colombo-Aleman de Invesligaciones Cientificas, 3, 29

10.2307/2862

Sergeeva T. K., 1975, The use of cross Immunoelectrophoresis for improving the sensitivity of the serological reaction when studying trophic relationships in insects. (In Russian, English Summary., Zoologicheskir zhurnal, 54, 1014

10.1017/S0007485300003862

Thông tin
Thông tin xuất bản

Annals of Applied Biology

Tập 90 Số 1

7-10

Thông tin tác giả