Một quy trình nhanh chóng, không xâm lấn để đánh giá định lượng khả năng sống sót trong điều kiện hạn hán bằng cách sử dụng huỳnh quang diệp lục
Tóm tắt
Phân tích khả năng sống sót thường được sử dụng như một phương tiện để so sánh hiệu suất của các dòng thực vật dưới điều kiện hạn hán. Tuy nhiên, việc đánh giá tình trạng nước của thực vật trong các nghiên cứu như vậy thường liên quan đến việc tách rời để ước lượng cú sốc nước, những phương pháp ước lượng không chính xác hoặc các phép đo xâm lấn như điều chỉnh thẩm thấu, ảnh hưởng đến hoặc hủy bỏ sự đánh giá tiếp theo về phản ứng của mẫu vật đối với hạn hán.
Bài báo này trình bày một quy trình để đánh giá nhanh chóng, giá rẻ và không xâm lấn khả năng sống sót của các cây trồng trên đất trong quá trình điều trị hạn hán. Những thay đổi trong các tham số quang hợp chính trong điều kiện thiếu nước ngày càng tăng đã được theo dõi thông qua hình ảnh huỳnh quang diệp lục và lựa chọn tham số hiệu suất tối đa của hệ thống quang hợp II (Fv/Fm) như một phương tiện đơn giản và thực tiễn nhất để theo dõi khả năng sống sót được mô tả. Tính hợp lệ của kỹ thuật này được xác minh thông qua ứng dụng với nhiều loại hình sinh thái và các dòng đột biến của
Từ khóa
#Hạn hán #khả năng sống sót #huỳnh quang diệp lục #<jats:italic>Arabidopsis thaliana</jats:italic> #đo không xâm lấnTài liệu tham khảo
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