A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress

Silvia Forcat1, Mark H. Bennett1, John W. Mansfıeld1, Murray Grant2
1Biology Division, Imperial College London, London, UK
2School of Biosciences, University of Exeter, Exeter, UK

Tóm tắt

Abstract We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.

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Tài liệu tham khảo

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