A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Springer Science and Business Media LLC - Tập 197 Số 2 - Trang 345-346 - 1984
Jef D. Boeke1, Francois La Croute2, Gerald R. Fink3
1Massachusetts Institute of Technology and Whitehead Institute for Biomedical Research, Cambridge, USA
2Laboratoire de Genetique Physiologique
3Whitehead Inst. for Biomedical Research

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Bach M, LaCroute F (1972) Direct selective techniques for the isolation of pyrimidine auxotrophs in yeast. Mol Gen Genet 115: 126–130

Bach M, LaCroute F, Botstein D (1979) Evidence for transcriptional regulation of orotidine-5′-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc Natl Acad Sci USA 76: 386–391

Botstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchomb D, Struhl K, Davis RW (1979) Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA materials. Gene 8: 17–24

Chattoo BB, Sherman F, Azubalis DA, Fjellstedt TA, Mehvert D, Ogur M (1979) Selection of lys2 mutants of the yeast Saccharomyces cerevisiae by the utilization of α-amino-adipate. Genetics 93: 51–65

Jund R, LaCroute F (1972) Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae. J Bacteriol 109: 196–202

Orr-Weaver TL, Szostak JW, Rothstein RJ (1983) Genetic applications of yeast transformation with linear and gapped plasmids. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology: Recombinant DNA, Part B. Academic Press, New York, p 228

Rose M, Winston F (1984) Identification of a Ty insertion within the coding sequence of the Saccharomyces cerevisiae URA3 gene. Mol Gen Genet 193: 557–560

Rose M, Grisafi P, Botstein D (1984) Structure and function of the yeast URA3 gene: Expression in E. coli. Gene, in press

Singh A, Sherman F (1974) Characteristics and relationships of mercury-resistant mutants and methionine auxotrophy of yeast. J Bacteriol 118: 911–918

Singh A, Sherman F (1975) Genetic and physiological characterization of met15 mutants of Saccharomyces cerevisiae: A selective system for forward and reverse mutations. Genetics 81: 75–97

Winston F, Chumley FG, Fink GR (1983) Eviction and transplacement of mutant genes in yeast. In: Wu R, Grossman L, Moldave D (eds) Methods in enzymology: Recombinant DNA, Part B. Academic Press, New York, p 211

Winston F, Chaleff DT, Valent B, Fink GR (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107: 179–197