A polyketide synthase gene required for ochratoxin A biosynthesis in Aspergillus ochraceus

Microbiology (United Kingdom) - Tập 149 Số 12 - Trang 3485-3491 - 2003
John O’Callaghan1, Mark X. Caddick2, Alan D. W. Dobson1
1Department of Microbiology, University College Cork, National University of Ireland Cork, Cork, Ireland
2School of Biological Sciences, University of Liverpool, Liverpool, UK

Tóm tắt

Ochratoxin A is an important nephrotoxic and nephrocarcinogenic mycotoxin, produced by Aspergillus ochraceus as a polyketide-derived secondary metabolite. A portion of a putative polyketide synthase gene (pks) involved in the biosynthesis of this mycotoxin was cloned by using a suppression subtractive hybridization PCR-based approach. The predicted amino acid sequence of the 1·4 kb clone shared 28–35 % identity to acyl transferase regions from fungal polyketide synthases found in the databases. Based on reverse transcription PCR studies, the pks gene is expressed only under ochratoxin A permissive conditions and only during the early stages of the mycotoxin synthesis. A mutant in which the pks gene has been interrupted cannot synthesize ochratoxin A. This report is the first of the cloning and characterization of a gene involved in ochratoxin A biosynthesis.

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Tài liệu tham khảo

Abarca, 1994, Ochratoxin A production by strains of Aspergillus niger var. niger, Appl Environ Microbiol, 60, 2650, 10.1128/AEM.60.7.2650-2652.1994

Abarca, 2001, Current importance of ochratoxin A-producing Aspergillus species, J Food Prot, 64, 903, 10.4315/0362-028X-64.6.903

Abe, 2002, Effect of increased dosage of the ML-236B (compactin) biosynthetic gene cluster on ML-236B production in Penicillium citrinum, Mol Genet Genomics, 268, 130, 10.1007/s00438-002-0736-8

Bayman, 2002, Ochratoxin production by the Aspergillus ochraceus group and Aspergillus alliaceus, Appl Environ Microbiol, 68, 2326, 10.1128/AEM.68.5.2326-2329.2002

Bullerman, 1987, Methods for detecting mycotoxins in foods and beverages, Food and Beverage Mycology, 571

Cabanes, 2002, What is the source of ochratoxin A in wine?, Int J Food Microbiol, 79, 213, 10.1016/S0168-1605(02)00087-9

Castella, 2002, Molecular characterization of ochratoxin A producing strains of the genus Penicillium, Syst Appl Microbiol, 25, 74, 10.1078/0723-2020-00094

Creppy, 2002, Update of survey, regulation and toxic effects of mycotoxins in Europe, Toxicol Lett, 127, 19, 10.1016/S0378-4274(01)00479-9

Cullen, 1987, Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph, Gene, 57, 21, 10.1016/0378-1119(87)90172-7

Dai, 2003, Ochratoxin A forms a carbon-bonded C8-deoxyguanosine nucleotide adduct: implications for C8 reactivity by a phenolic radical, J Am Chem Soc, 125, 3716, 10.1021/ja034221r

Dalcero, 2002, Detection of ochratoxin A in animal feeds and capacity to produce this mycotoxin by Aspergillus section Nigri in Argentina, Food Addit Contam, 19, 1065, 10.1080/02652030210151895

Diatchenko, 1996, Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries, Proc Natl Acad Sci U S A, 93, 6025, 10.1073/pnas.93.12.6025

Edwards, 2002, PCR-based detection and quantification of mycotoxigenic fungi, Mycol Res, 106, 1005, 10.1017/S0953756202006354

Feng, 1995, Characterization of the polyketide synthase gene ( pksL1 ) required for aflatoxin biosynthesis in Aspergillus parasiticus, J Bacteriol, 177, 6246, 10.1128/JB.177.21.6246-6254.1995

Gareis, 2000, Ochratoxin A in meat and meat products, Arch Lebensmittelhyg, 51, 102

Heenan, 1998, Ochratoxin A production by Aspergillus carbonarius and A. niger isolates and detection using coconut cream agar, J Food Mycol, 1, 76

Hendrickson, 1999, Lovastatin biosynthesis in Aspergillus terreus : characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene, Chem Biol, 6, 429, 10.1016/S1074-5521(99)80061-1

Hussein, 2001, Toxicity, metabolism, and impact of mycotoxins on humans and animals, Toxicology, 167, 101, 10.1016/S0300-483X(01)00471-1

Jorgensen, 2002, Occurrence of ochratoxin A in Danish wheat and rye, 1992–1999, Food Addit Contam, 19, 1184, 10.1080/0265203021000012376

Lindsey, 2002, Ochratoxin A may cause testicular cancer, Lancet Oncol, 3, 129, 10.1016/S1470-2045(02)00665-4

Maaroufi, 1995, Foodstuffs and human blood contaminated by the mycotoxin ochratoxin A: correlation with chronic interstitial nephropathy in Tunisia, Arch Toxicol, 69, 552, 10.1007/s002040050211

Moss, 1996, Mode of formation of ochratoxin A, Food Addit Contam, 13, 5

Moss, 1998, Recent studies of mycotoxins, Symp Ser Soc Appl Microbiol, 27, 62S, 10.1046/j.1365-2672.1998.0840s162S.x

Muller, 2003, Effects of the mycotoxin ochratoxin A and some of its metabolites on the human cell line THP-1, Toxicology, 184, 69, 10.1016/S0300-483X(02)00593-0

Petziner, 2000, Ochratoxin A from a toxicological perspective, J Vet Pharmacol Ther, 23, 91, 10.1046/j.1365-2885.2000.00244.x

Pitt, 2000, Toxigenic fungi: which are important?, Med Mycol, 38, 17, 10.1080/mmy.38.s1.17.22

Proctor, 2003, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genet Biol, 38, 237, 10.1016/S1087-1845(02)00525-X

Schuster, 2002, On the safety of Aspergillus niger – a review, Appl Microbiol Biotechnol, 59, 426, 10.1007/s00253-002-1032-6

Shyamala, 1989, Genome walking by single-specific-primer polymerase chain reaction: SSP-PCR, Gene, 84, 1, 10.1016/0378-1119(89)90132-7

Sibanda, 2002, Optimization of solid-phase clean-up prior to liquid chromatographic analysis of ochratoxin A in roasted coffee, J Chromatogr A, 959, 327, 10.1016/S0021-9673(02)00408-9

Soden, 2001, Differential regulation of laccase gene expression in Pleurotus sajor-caju, Microbiology, 147, 1755, 10.1099/00221287-147-7-1755

Southern, 1992, Detection of specific sequences among DNA fragments separated by gel electrophoresis, Biotechnology, 24, 122

Stefanaki, 2003, Ochratoxin A concentrations in Greek domestic wines and dried vine fruit, Food Addit Contam, 20, 74, 10.1080/0265203021000031537

Tang, 1992, An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity, Mol Microbiol, 6, 1663, 10.1111/j.1365-2958.1992.tb00891.x

Taniwaki, 2003, The source of ochratoxin A in Brazilian coffee and its formation in relation to processing methods, Int J Food Microbiol, 82, 173, 10.1016/S0168-1605(02)00310-0

Tilburn, 1995, The Aspergillus PacC zinc finger transcription factor mediates regulation of both acidic- and alkaline-expressed genes by ambient pH, EMBO J, 14, 779, 10.1002/j.1460-2075.1995.tb07056.x

van der Merwe, 1965, Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh, Nature, 205, 1112, 10.1038/2051112a0

Visconti, 2000, Determination of ochratoxin A in domestic and imported beers in Italy by immunoaffinity clean-up and liquid chromatography, J Chromatogr A, 888, 321, 10.1016/S0021-9673(00)00549-5

Walker, 2002, Risk assessment of ochratoxin: current views of the European Scientific Committee on Food, the JECFA and the Codex Committee on Food Additives and Contaminants, Adv Exp Med Biol, 504, 249, 10.1007/978-1-4615-0629-4_26

Yang, 1996, A polyketide synthase is required for fungal virulence and production of the polyketide T-toxin, Plant Cell, 8, 2139