A novel approach to quantitative analysis of electrophoretic gel images of DNA fragments

L. Bajla1, L. Hollander1, K. Burg2, S. Fluch2
1Unit of High Performance Image Processing, ARC Seibersdorf research GmbH, Austria
2Unit of Biotechnology, ARC Seibersdorf research GmbH, Austria

Tóm tắt

A novel methodology of DNA gel image analysis has been proposed that is based on 2D image processing methods instead of previously used 1D Gaussian deconvolution. The algorithms specifically tailored to band boundary detection and image intensity homogeneity characterization have been developed. The proposed methodology involves a modified type of user interaction.

Từ khóa

#Image analysis #DNA #Deconvolution #Image processing #Software systems #Biotechnology #Electrokinetics #Information analysis #Integral equations #Convolution

Tài liệu tham khảo

sen, 1971, Nonparametric Methods in Multivariate Analysis 10.1007/s001380050076 ch, 2000, Numerical aspects of Deconvolution Dept of Math Modelling TV of Denmark