A nomenclature for intestinal in vitro cultures

American Journal of Physiology - Gastrointestinal and Liver Physiology - Tập 302 Số 12 - Trang G1359-G1363 - 2012
Matthias Stelzner1, Michael A. Helmrath2, James Dunn1, Susan Henning3, Courtney W. Houchen4, Calvin J. Kuo5, John P. Lynch6, Linheng Li7, Scott T. Magness3, Martı́n G. Martı́n1, Melissa H. Wong8, Jian Yu9
1University of California Los Angeles, Los Angeles, California
2University of Cincinnati, Cincinnati, Ohio
3University of North Carolina, Chapel Hill, North Carolina
4University of Oklahoma, Oklahoma City, Oklahoma
5Stanford University, Stanford, California
6University of Pennsylvania, Philadelphia, Pennsylvania
7Stowers Institute for Medical Research, Kansas City, Missouri
8Oregon Health and Sciences University, Portland, Oregon; and
9University of Pittsburgh, Pittsburgh, Pennsylvania

Tóm tắt

Many advances have been reported in the long-term culture of intestinal mucosal cells in recent years. A significant number of publications have described new culture media, cell formations, and growth patterns. Furthermore, it is now possible to study, e.g., the capabilities of isolated stem cells or the interactions between stem cells and mesenchyme. However, at the moment there is significant variation in the way these structures are described and named. A standardized nomenclature would benefit the ability to communicate and compare findings from different laboratories using the different culture systems. To address this issue, members of the NIH Intestinal Stem Cell Consortium herein propose a systematic nomenclature for in vitro cultures of the small and large intestine. We begin by describing the structures that are generated by preparative steps. We then define and describe structures produced in vitro, specifically: enterosphere, enteroid, reconstituted intestinal organoid, induced intestinal organoid, colonosphere, colonoid, and colonic organoid.

Từ khóa


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Thông tin xuất bản

American Journal of Physiology - Gastrointestinal and Liver Physiology

Tập 302 Số 12

G1359-G1363

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