A marine diatom-derived aldehyde induces apoptosis in copepod and sea urchin embryos
Tóm tắt
The diatom-derived aldehyde 2-trans-4-trans-decadienal(DD) was tested as an apoptogenic inducer in both copepod and sea urchin embryos, using terminal-deoxynucleotidyl-transferase-mediated dUTP nick-end labelling (TUNEL), DNA fragmentation profiling (laddering) and an assay for caspase-3 activity. DD induced TUNEL positivity and DNA laddering, but not caspase-like activation, in copepod embryos spawned by females fed for 10-15 days the diatom diet Thalassiosira rotula Meunier (in vivo),or when newly spawned eggs were exposed for 1 h to 5 μg ml-1 DD(in vitro). To our knowledge, this is the first time that evidence for an apoptotic process in copepods has been obtained by cytochemical (TUNEL)and biochemical (DNA fragmentation) approaches. The absence of caspase-like activity in copepod embryos suggests that caspase-independent programmed cell death occurs in these organisms. In sea urchin embryos, DD induced apoptosis and also activated a caspase-3-like protease. The saturated aldehyde decanal induced apoptosis at higher concentrations and after a longer incubation period than DD, indicating that α,β-unsaturation of the molecule,coupled with the aldehyde group, is responsible for the greater biological activity of DD. Since diatoms are an important food source for marine herbivores such as copepods and sea urchins, these findings may help explain why unsaturated aldehydes often induce reproductive failure, with important ecological consequences at the population level.
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Tài liệu tham khảo
Adolph, S., Poulet, S. A. and Pohnert, G.(2003). Synthesis and biological activity of alpha,beta,gamma,delta-unsaturated aldehydes from diatoms. Tetrahedron59,3003-3008.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem.72,248-254.
Bruenner, B. A., Jones, A. D. and German, J. B.(1994). Maximum-entropy deconvolution of heterogeneity in protein modification - protein adducts of 4-hydroxy-2-nonenal. Rapid Commun. Mass Spect.8,509-512.
Bucklin, A., Frost, B. W. and Kocher, T. D.(1995). Molecular systematics of six Calanus and three Metridia species (Calanoida: Copepoda). Mar. Biol.121,655-664.
Buszczak, M. and Cooley, L. (2000). Eggs to die for: cell death during Drosophila oogenesis. Cell Death Differ.7,1071-1074.
Buttino, I., Miralto, A., Ianora, A., Romano, G. and Poulet, S. A. (1999). Water-soluble extracts of the diatom Thalassiosira rotula induce aberrations in embryonic tubulin organisation of the sea urchin Paracentrotus lividus. Mar. Biol.134,147-154.
Chaudron, Y., Poulet, S. A., Laabir, M., Ianora, A. and Miralto,A. (1996). Is hatching success of copepod eggs diatom density-dependent? Mar. Ecol. Prog. Ser144,185-193.
d'Ippolito, G., Romano, G., Caruso, T., Spinella, A., Cimino, G. and Fontana, A. (2003). Production of octadienal in the marine diatom Skeletonema costatum. Organic Lett.5,885-887.
Hamilton, R. F., Li, L., Eschenbacher, W. L., Szweda, L. and Holian, A. (1998). Potential involvement of 4-hydroxynonenal in the response of human lung cells to ozone. Am. J. Physiol. Lung Cell. Mol. Physiol.18,L8-L16.
Ianora, A., Poulet, S. A. and Miralto, A.(2003). The effects of diatoms on copepod reproduction: a review. Phycologia42,351-363.
Jacobson, M., Weil, M. and Raff, M. (1997). Programmed cell death in animal development. Cell88,347-354.
Johnson, K. L., Grubb, D. R. and Lawen, A.(1999). Unspecific activation of caspases during the induction of apoptosis by didemnin B in human cell lines. J. Cell. Biochem.72,269-278.
Kirichenko, A., Li, L., Morandi, M. T. and Holian, A.(1996). 4-Hydroxy-2-nonenal-protein adducts and apoptosis in murine lung cells after acute ozone exposure. Toxicol. Appl. Pharmacol.141,416-424.
Kruman, I., BruceKeller, A. J., Bredesen, D., Waeg, G. and Mattson, M. P. (1997). Evidence that 4-hydroxynonenal mediates oxidative stress-induced neuronal apoptosis. J. Neurosci.17,5089-5100.
Lindeque, P. K., Harris, R. P., Jones, M. B. and Smerdon, G. R. (1999). Simple molecular method to distinguish the identity of Calanus species (Copepoda: Calanoida) at any developmental stage. Mar. Biol.133, 91-96.
Lockshin, R. A., Zakeri, Z. and Tilly, J. L.(1998). When Cells Die: A Comprehensive Evaluation of Apoptosis and Programmed Cell Death. New York:Wiley-Liss.
Mathiasen, I. S. and Jaattela, M. (2002). Triggering caspase-independent cell death to combat cancer. Trends Mol. Med.8,212-220.
Matsui, K., Kurishita, S., Hisamitsu, A. and Kajiwara, T.(2000). A lipidhydrolysing activity involved in hexenal formation. Biochem. Soc. Trans.28,857-860.
Miralto, A., Barone, G., Romano, G., Poulet, S. A., Ianora, A.,Russo, G. L., Buttino, I., Mazzarella, G., Laabir, M., Cabrini, M. et al., (1999). The insidious effect of diatoms on copepod reproduction. Nature402,173-176.
Miralto, A., Guglielmo, L., Zagami, G., Buttino, I., Granata, A. and Ianora, A. (2003). Inhibition of population growth in the copepods Acartia clausi and Calanus helgolandicusduring diatom blooms. Mar. Ecol. Prog. Ser254,253-268.
Morita, Y. and Tilly, J. L. (1999). Oocyte apoptosis: like sand through an hourglass. Dev. Biol.213, 1-17.
Pohnert, G. (2000). Wound-activated chemical defense in unicellular planktonic algae. Angew. Chem. Int. Edit.39,4352-4354.
Pohnert, G. (2002). Phospholipase A(2) activity triggers the wound-activated chemical defense in the diatom Thalassiosira rotula. Plant Physiol.129,103-111.
Pohnert, G. and Boland, W. (1996). Biosynthesis of the algal pheromone hormosirene by the freshwater diatom Gomphonema parvulum (Bacillariophyceae). Tetrahedron52,10073-10082.
Pohnert, G. and Boland, W. (2002). The oxylipin chemistry of attraction and defense in brown algae and diatoms. Nat. Prod. Rep.19,108-122.
Pohnert, G., Lumineau, O., Cueff, A., Adolph, S., Cordevant, C.,Lange, M. and Poulet, S. (2002). Are volatile unsaturated aldehydes from diatoms the main line of chemical defence against copepods? Mar. Ecol. Prog. Ser.245, 33-45.
Poulet, S. A., Ianora, A., Miralto, A. and Meijer, L.(1994). Do diatoms arrest embryonic development in copepods?Mar. Ecol. Prog. Ser.111, 79-86.
Refsgaard, H. H. F., Tsai, L. and Stadtman, E. R.(2000). Modifications of proteins by polyunsaturated fatty acid peroxidation products. Proc. Natl. Acad. Sci. USA97,611-616.
Rosahl, S. (1996). Lipoxygenases in plants -Their role in development and stress response. Z. Naturforsch.51c,123-138.
Spiteller, G. (2001). Lipid peroxidation in aging and age-dependent diseases. Exp. Gerontol.36,1425-1457.
Staley, K., Blaschke, A. J. and Chun, J.(1997). Apoptotic DNA fragmentation is detected by a semiquantitative ligation-mediated PCR of blunt DNA ends. Cell Death Differ.4,66-75.
Tang, D. G., La, E. H., Kern, J. and Kehrer, J. P.(2002). Fatty acid oxidation and signaling in apoptosis. Biol. Chem.383,425-442.
Tilly, J. L. (1998). Molecular and genetic basis of normal and toxicant-induced apoptosis in female germ cells. Toxicol. Lett.102-103,497-501.
Tosti, E., Romano, G., Buttino, I., Cuomo, A., Ianora, A. and Miralto, A. (2003). Bioactive aldehydes from diatoms block the fertilization current in asciadian oocytes. Mol. Reprod. Dev.66,72-80.
Turner, J. T., Ianora, A., Miralto, A., Laabir, M. and Esposito,F. (2001). Decoupling of copepod grazing rates, fecundity and egg-hatching success on mixed and alternatine diatom and dinoflagellate diets. Mar. Ecol. Prog. Ser.220,187-199.