A magnetizable solid phase for enzyme extraction

Applied Biochemistry and Biotechnology - Tập 30 - Trang 155-164 - 1991
Michael P. Ennis1, G. Brian Wisdom1
1Division of Biochemistry, The Queen s University of Belfast, Medical Biology Centre, Belfast, UK

Tóm tắt

A method for the convenient and reliable preparation of magnetizable agarose beads containing iron particles is described. The beads were treated with the triazine dye, Reactive Red 120, and the matrix was examined for the ability to extract proteins from crude preparations using lactate dehydrogenase from porcine muscle as a model. The recovery and specific activity values of enzyme obtained using this matrix and magnetic field separation were significantly greater than those for enzyme purified by centrifugation and conventional dye ligand chromatography.

Tài liệu tham khảo

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