A high throughput, interactive imaging, bright‐field wound healing assay

Cytometry. Part A : the journal of the International Society for Analytical Cytology - Tập 79A Số 3 - Trang 227-232 - 2011
Michael D. Zordan1,2,3, Christopher P. Mill4,5,6,7, David J. Riese4,1,5,6,7, James F. Leary1,2,8,7,9,3
1Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907
2Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907
3Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907
4Auburn University, Auburn, Alabama 36849
5Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, West Lafayette, Indiana 47907
6Harrison School of Pharmacy
7Purdue Center for Cancer Research, West Lafayette, Indiana 47907
8Department of Basic Medical Science, School of Veterinary Medicine
9Purdue University, West Lafayette, Indiana, 47907

Tóm tắt

AbstractThe wound healing assay is a commonly used technique to measure cell motility and migration. Traditional methods of performing the wound healing assay suffer from low throughput and a lack of quantitative data analysis. We have developed a new method to perform a high‐throughput wound healing assay that produces quantitative data using the LEAP™ instrument. The LEAP™ instrument is used to create reproducible wounds in each well of a 96‐well plate by laser ablation. The LEAP™ then records bright field images of each well at several time points. A custom texture segmentation algorithm is used to determine the wound area of each well at each time point. This texture segmentation analysis can provide faster and more accurate image analysis than traditional methods. Experimental results show that reproducible wounds are created by laser ablation with a wound area that varies by less than 10%. This method was tested by confirming that neuregulin‐2β increases the rate of wound healing by MCF7 cells in a dose dependent manner. This automated wound healing assay has greatly improved the speed and accuracy, making it a suitable high‐throughput method for drug screening. © 2011 International Society for Advancement of Cytometry

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Thông tin xuất bản

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Tập 79A Số 3

227-232

Thông tin tác giả