A disposable membrane strip immunosensor
SENSORS, 2002 IEEE - Tập 1 - Trang 312-317 vol.1
Tóm tắt
As the safety in the food supply becomes critical, the demand for rapid, low volume, sensitive, and economic biosensing devices has dramatically increased. An immunosensor based on electrochemical sandwich immunoassay using polyaniline has been developed for detecting foodborne pathogens, such as Escherichia coli 0157:H7. The immunosensor design is based primarily upon the specific nature of labeled antibody-antigen binding. The architecture of the biosensor demonstrates the advantages of using lateral flow formal strip attached to a portable circuitry for signal measurement. The ability to change the specificity of the antibodies enables the biosensor to be used as a semi-quantitative detection device for other types of foodborne pathogens. Results show that the biosensor could detect as low as 10/sup 1/ bacterial cells per milliliter in less than 10 minutes. Additionally, the biosensor yields faster results, and is cheaper than conventional approaches. Details on the device fabrication and performance of the biosensor in detecting E. coli 0157:H7 are presented.
Từ khóa
#Biomembranes #Strips #Biosensors #Immune system #Pathogens #Safety devices #Circuits #Fluid flow measurement #Microorganisms #FabricationTài liệu tham khảo
10.1016/S0013-4686(96)00360-X
10.1016/S0379-6779(96)04144-6
griffin, 1991, The Epidemiology of Infections Caused by Escherichia coli 0157:H7, print Enterohemorrhagic E. coli, and the Associated Hemolytic Uremic Syndrome, Epidemiology Review, 13, 60, 10.1093/oxfordjournals.epirev.a036079
10.1007/978-94-011-3396-8_5
10.1021/ac00045a031
10.1097/00000441-199312000-00009
imisides, 1996, Microsensors based on conducting polymers, 26, 19
ivnitski, 1999, Biosensor & Bioelectronics, 14, 599
10.1007/978-1-4615-4427-2
10.1016/S0956-5663(99)00063-9
brunelle, 2001, Electroimmunoassay technology for Foodborne pathogen detection, Detection Technology, 55
beesley, 1993, Immunocytochemistry; A practical Approach, 151
cahn, 1993, Biosensors
buchanan, 1997, Foodborne Disease Significance of Escherichia coli 0157:H7 and print Enterohemorrhagic E. coli, Food Technology, 51, 69
10.1016/0379-6779(86)90070-6
10.1016/0302-4598(95)01894-8
0, Bacteriological Analytical Manual 8th Edition, US Food and Drug Administration
d'souza, 2001, Microbial Biosensors (Review) Biosensor & Bioelectronics, 16, 337
10.1016/S0379-6779(97)80659-5
1999, A short guide: Developing Immunochromatographic Test Strips, Millipore 2nd Edition
10.1016/S0956-5663(97)00039-0
10.1016/S0379-6779(98)00107-6
10.1016/0039-9140(91)80261-W
10.1016/S0925-4005(97)80006-8
yin, 1997, Experimental evidence for hydrogen bonding in polyaniline: Mechanism of aggregate formation and dependency on oxidation state, Macromolecules, 30, 2953, 10.1021/ma9700136
turner, 1998, An Introduction to Biosensor, in A.O. Scott (ed), Biosensor for Food Analysis, 13