A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii

Genes and Development - Tập 21 Số 10 - Trang 1190-1203 - 2007
Tao Zhao1, Guanglin Li2, Shijun Mi1, Shan Li1, Gregory J. Hannon3, Xiu‐Jie Wang2, Yijun Qi1
1National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
2State Key Laboratory of Plant Genomics , Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
3Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA

Tóm tắt

Endogenous small RNAs function in RNA interference (RNAi) pathways to control gene expression through mRNA cleavage, translational repression, or chromatin modification. Plants and animals contain many microRNAs (miRNAs) that play vital roles in development, including helping to specify cell type and tissue identity. To date, no miRNAs have been reported in unicellular organisms. Here we show that Chlamydomonas reinhardtii, a unicellular green alga, encodes many miRNAs. We also show that a Chlamydomonas miRNA can direct the cleavage of its target mRNA in vivo and in vitro. We further show that the expression of some miRNAs/Candidates increases or decreases during Chlamydomonas gametogenesis. In addition to miRNAs, Chlamydomonas harbors other types of small RNAs including phased small interfering RNAs (siRNAs) that are reminiscent of plant trans-acting siRNAs, as well as siRNAs originating from protein-coding genes and transposons. Our findings suggest that the miRNA pathway and some siRNA pathways are ancient mechanisms of gene regulation that evolved prior to the emergence of multicellularity.

Từ khóa


Tài liệu tham khảo

10.1038/ng1478

10.1016/j.cell.2005.04.004

10.1016/S1534-5807(03)00228-4

Aravin,, 2006, A novel class of small RNAs bind to MILI protein in mouse testes, Nature, 442, 203, 10.1038/nature04916

10.1105/tpc.105.032185

10.1016/j.cell.2006.09.032

10.1016/S0092-8674(04)00045-5

10.1073/pnas.0505461102

10.1016/j.cell.2005.11.035

10.1038/nsmb729

10.1101/gad.1026102

10.1126/science.1085242

10.1101/gad.862301

10.1016/j.cub.2005.07.024

Girard,, 2006, A germline-specific class of small RNAs binds mammalian Piwi proteins, Nature, 442, 199, 10.1038/nature04917

10.1093/nar/gki081

10.1101/gad.1434406

10.1128/EC.2.6.1137-1150.2003

10.1093/emboj/cdf464

10.1038/418244a

Harris, E.H. (1989) The Chlamydomonas sourcebook: A comprehensive guide to biology and laboratory use (Academic Press, New York).

10.1073/pnas.022392999

10.1016/j.molcel.2004.05.027

10.1146/annurev.arplant.57.032905.105218

10.1159/000084979

10.1101/gr.229202. Article published online before March 2002

10.1186/1471-2105-7-474

10.1126/science.1065062

10.1126/science.1130164

10.1101/gad.1377006

Lefebvre,, 1999, Chlamydomonas: The cell and its genomes, Genetics, 151, 9, 10.1093/genetics/151.1.9

10.3732/ajb.91.10.1535

10.1038/nature02875

10.1126/science.1076311

10.1126/science.1114112

Margulies,, 2005, Genome sequencing in microfabricated high-density picolitre reactors, Nature, 437, 376, 10.1038/nature03959

10.1038/nature02873

10.1101/gad.1219904

10.1016/j.gde.2004.01.004

10.1016/j.cell.2004.11.034

10.3732/ajb.91.10.1437

10.1101/gad.1231804

10.1016/j.molcel.2005.06.014

10.1038/nature05198

10.1101/gad.1476406

10.1038/nsmb918

10.1007/s00294-005-0042-1

10.1104/pp.016899

10.1073/pnas.0407641102

10.1111/j.1365-313X.2006.02895.x

10.1126/science.1129333

10.1101/gad.1410506

10.1016/j.molcel.2004.09.028

10.1186/gb-2004-5-9-r65

10.1104/pp.90.2.512

10.1126/science.290.5494.1159

10.1371/journal.pbio.0020104

10.1073/pnas.0506426102

10.1146/annurev.genet.39.073003.095906

10.1126/science.1097434

10.1101/gad.1352605

10.1046/j.1365-313X.2002.01331.x

10.1126/science.1079695

10.1093/nar/gkg595

Thông tin
Thông tin xuất bản

Genes and Development

Tập 21 Số 10

1190-1203

Thông tin tác giả