A comparison of aromatase, 5α‐, and 5β‐ reductase activities in the brain and pituitary of male and female quail <i>(C. c. japonica)</i>

Wiley - Tập 242 Số 2 - Trang 171-180 - 1987
Barney A. Schlinger1, Gloria V. Callard1
1Department of Biological Sciences, Boston University, Boston, Massachusetts 02215

Tóm tắt

AbstractIn numerous vertebrate species including Japanese quail (Co‐turnix coturnix japonica), actions of testosterone (T) on neuroendocrine target tissues are mediated in part by conversion to estrogenic and androgenic metabolites. In order to assess which pathways were favored in each identified androgen target area in quail brain and whether there were discernible sex differences, we developed an assay for simultaneously quantifying aromatase, 5α‐, and 5β‐reductase. In addition, we made the first definitive identification of aromatase in quail pituitary and compared all three enzyme activities in the pituitary of males and females. Enzymes were measured in tissue homogenates by the conversion of [3H]androstenedione to [3H]estrone, [3H]5α‐androstanedione, and 5β‐androstanedione. Aromatase activity was restricted to limbic tissues (anterior hypothalamus > posterior hypothalamus > septum > archistriatum containing nucleus taenia) while hyperstriatum, cerebellum, and midbrain containing nucleus intercollicularis were aromatase‐negative. Quail pituitary aromatized androgen at rates equivalent to anterior hypothalamus pre‐optic area (aHPOA). 5α‐ and 5β‐reductase were present in all tissues tested. Aromatase was significantly higher in aHPOA and pituitary of males, whereas 5α‐reductase was significantly higher in female pituitary. These data suggest that a complex of androgen‐metabolizing enzymes controls the neuroanatomic (spatial) distribution of active hormone in neuroendocrine tissues and that quantitative differences between males and females may account for sex differences in behavior.

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Tài liệu tham khảo

10.1016/0031-9384(73)90232-1

10.1016/0031-9384(80)90233-4

10.1016/0016-6480(83)90072-2

10.1016/0018-506X(69)90004-X

10.1210/endo-89-6-1470

10.1159/000122785

Bayle J. D., 1974, Stereotaxic topography of the brain of the quail, J. Physiol. (Paris), 68, 219

Callard G. V., 1984, Metabolism of Hormonal Steroids in the Neuroendocrine Structures, 79

10.1210/endo-103-6-2283

10.1095/biolreprod21.1.33

10.1210/endo-113-1-152

10.1095/biolreprod35.2.282

10.1016/0006-8993(86)91135-2

Connolly P.(1986)Control of gonadotropin secretion from the pituitary of the Japanese quail in vitro.Ph. D. Dissertation Boston University Boston.

10.1677/joe.0.0840211

10.1159/000122914

10.1677/joe.0.0780267

Holzbauer M., 1976, Physiological aspects of steroids with anaesthetic properties, Med. Biol., 54, 227

10.1038/216591a0

10.1126/science.7244635

10.1016/S0079-6123(08)64427-1

10.1210/endo-118-6-2180

10.1677/joe.0.0740425

10.1007/BF00215122

10.1093/icb/18.3.425

10.1111/j.1471-4159.1983.tb13591.x

10.1016/0300-9629(86)90711-5

10.1073/pnas.58.3.985

10.1210/endo-100-2-588

10.1210/endo-97-3-517

10.1016/S0021-9258(19)52451-6

10.1016/0016-6480(80)90152-5

10.1073/pnas.83.2.513

10.1210/edrv-3-1-1

10.1677/joe.0.0750347

10.1677/joe.0.0880263

10.1146/annurev.ne.02.030179.000433

10.1126/science.6259728

Naftolin F., 1975, The formation of estrogens by central neuroendocrine tissues, Rec. Prog. Horm. Res., 31, 255

Naftolin F., 1975, The formation and metabolism of estrogens in brain tissues, Adv. Biosci., 15, 107

Nakamura T., 1974, In vitro metabolism of steroid hormones by chicken brain, Acta. Endocrinol., 75, 410

10.1002/cne.901650405

10.1016/0016-6480(85)90344-2

10.1016/0016-6480(85)90320-X

10.1210/endo-114-1-192

10.1016/0022-4731(77)90063-2

Schlinger B., 1984, Distribution of aromatase activity in quail (Coturnix coturnix japonica) brain: effects of photoperiod and castration, J. Ster. Biochem., 1571

10.1016/0006-8993(84)91118-1

Schumacher M., 1986, Testosterone induced brain aromatase is sexually dimorphic, Brain Res., 370, 285, 10.1016/0006-8993(86)90483-X

10.1677/joe.0.1080129

10.1016/0006-8993(83)90540-1

10.1016/0016-6480(72)90004-4

10.1210/endo-116-5-1869

10.1016/0016-6480(81)90336-1

10.1016/0304-3940(86)90087-X

10.1016/0018-506X(82)90015-0

10.1016/0018-506X(84)90038-2

10.1126/science.179.4077.1005

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Wiley

Tập 242 Số 2

171-180

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