A Versatile Vector Toolkit for Functional Analysis of Rice Genes

Rice - Tập 11 Số 1 - Trang 1-10 - 2018
He, Feng1,2, Zhang, Fan1, Sun, Wenxian2, Ning, Yuese1, Wang, Guo-Liang1,3
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
2College of Plant Protection, China Agricultural University, Beijing, China
3Department of Plant Pathology, The Ohio State University, Columbus, USA

Tóm tắt

Rice (Oryza sativa) is the main food for half of the world’s population, and is considered the model for molecular biology studies of monocotyledon species. Although the rice genome was completely sequenced about 15 years ago, the function of most rice genes is still unknown. In this study, we developed a vector toolkit that contains 42 vectors for transient expression studies in rice protoplasts and stable expression analysis in transgenic rice. These vectors have been successfully used to study protein subcellular localization, protein-protein interaction, gene overexpression, and the CRISPR/Cas9-mediated gene editing. A novel feature of these vectors is that they contain a universal multiple cloning site, which enables more than 99% of the rice coding sequences to be conveniently transferred between vectors. The versatile vectors represent a highly efficient and high-throughput toolkit for functional analysis of rice genes.

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