A Single-Laboratory Validated Method for the Generation of DNA Barcodes for the Identification of Fish for Regulatory Compliance

Journal of AOAC International - Tập 94 Số 1 - Trang 201-210 - 2011
Sara M. Handy1, Jonathan R. Deeds1, Natalya Ivanova2, Paul D. N. Hebert2, Robert Hanner3, Andrea Ormos4, Lee A. Weigt4, Michelle Moore5, Haile F. Yancy6
1U.S. Food and Drug Administration, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, College Park, MD 20740, USA
2Canadian Centre for DNA Barcoding, Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd East, Guelph, ON, Canada, N1G 2W1
3University of Guelph, Department of Integrative Biology, 50 Stone Rd East, Guelph, ON, Canada, N1G 2W1
4Smithsonian Institution, National Museum of Natural History, Laboratories of Analytical Biology, MRC 534, Washington, DC 20013-7012, USA
5U.S. Food and Drug Administration, Office of Regulatory Affairs, Pacific Regional Laboratory Northwest, Applied Technology Center, 22201 23rd Dr SE, Bothell, WA 98021-4421, USA
6U.S. Food and Drug Administration, Office of Research, Center for Veterinary Medicine, 8401 Muirkirk Rd, Laurel, MD 20708

Tóm tắt

Abstract The U.S. Food and Drug Administration is responsible for ensuring that the nation's food supply is safe and accurately labeled. This task is particularly challenging in the case of seafood where a large variety of species are marketed, most of this commodity is imported, and processed product is difficult to identify using traditional morphological methods. Reliable species identification is critical for both foodborne illness investigations and for prevention of deceptive practices, such as those where species are intentionally mislabeled to circumvent import restrictions or for resale as species of higher value. New methods that allow accurate and rapid species identifications are needed, but any new methods to be used for regulatory compliance must be both standardized and adequately validated. "DNA barcoding" is a process by which species discriminations are achieved through the use of short, standardized gene fragments. For animals, a fragment (655 base pairs starting near the 5′ end) of the cytochrome c oxidase subunit 1 mitochondrial gene has been shown to provide reliable species level discrimination in most cases. We provide here a protocol with single-laboratory validation for the generation of DNA barcodes suitable for the identification of seafood products, specifically fish, in a manner that is suitable for FDA regulatory use.

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Journal of AOAC International

Tập 94 Số 1

201-210

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