A Simple and General Method for Transferring Genes into Plants

American Association for the Advancement of Science (AAAS) - Tập 227 Số 4691 - Trang 1229-1231 - 1985
Robert B. Horsch1, J. E. Fry1, Nancy Hoffmann1, Marco Wallroth1, David A. Eichholtz1, Stephen L. Rogers1, Robert T. Fraley1
1Biological Sciences, Monsanto Company, St. Louis, Missouri 63167

Tóm tắt

Transformed petunia, tobacco, and tomato plants have been produced by means of a novel leaf disk transformation-regeneration method. Surface-sterilized leaf disks were inoculated with an Agrobacterium tumefaciens strain containing a modified tumor-inducing plasmid (in which the phytohormone biosynthetic genes from transferred DNA had been deleted and replaced with a chimeric gene for kanamycin resistance) and cultured for 2 days. The leaf disks were then transferred to selective medium containing kanamycin. Shoot regeneration occurred within 2 to 4 weeks, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.

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American Association for the Advancement of Science (AAAS)

Tập 227 Số 4691

1229-1231

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