A Simple and Effective Chemical Phosphorylation Procedure for Biomolecules

Helvetica Chimica Acta - Tập 70 Số 1 - Trang 175-186 - 1987
Willi Bannwarth1, Arnold Trzeciak1
1F. Hoffmann-La Roche & Co., Ltd., Central Research Units, CH–4002 Basel

Tóm tắt

Abstract

A general chemical phosphorylation method based on P(III) chemistry has been developed. The system is demonstrated for the phosphorylation of oligonucleotides, directly after their synthesis on a solid support, and for the O‐phosphorylation of serine, threonine, and tyrosine as well as for a serine‐containing peptide.

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