A Simple and Effective Chemical Phosphorylation Procedure for Biomolecules
Tóm tắt
A general chemical phosphorylation method based on P(III) chemistry has been developed. The system is demonstrated for the phosphorylation of oligonucleotides, directly after their synthesis on a solid support, and for the
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Tài liệu tham khảo
H.Boudjebel H.Conçalves F.Mathis Bull. Soc. Chim. Fr.1975 628.
B. J.Hunt W.Rigby Chem. Ind. (London)1967 1868.
Josephson S., 1981, Nucleic Acids Res. Symp. ser., 9, 177
Atherton F. R., 1957, Biochem. Prep., 5, 1
Avaeva S. M., 1964, Zh. Obshch. Khim., 34, 1749
Fölsch F., 1967, Sven. Kem. Tidskr., 79, 38
Korobka V. G., 1980, Nucleic Acids. Res. Symp. Ser., 7, 365
Wünsch E., 1974, Houben‐Weyl‐Müller, Methoden der organischen Chemie
Maniatis T., 1982, Molecular Cloning
D.Gillessen private communication (F. Hoffmann‐La Roche & Co. Ltd. Basel).