A Simple Hemagglutination System Requiring Small Amounts of Red Cells and Antibodies

Transfusion - Tập 6 Số 1 - Trang 67-73 - 1966
Thomas Wegmann1,2, Oliver Smithies1,3
1Department of Medical Genetics, University of Wisconsin, Madison, Wisconsin
2Thomas G. Wegmann, B.A., Graduate Student and Medical Student.
3Oliver Smithies, M.A., D.Phil., Professor of Genetics, University of Wisconsin, Madison, Wisc.

Tóm tắt

A method is described which permits dilute red cell suspensions (1/32%) to be used in plastic microtiter plates for detecting hemagglutinins at unusually high dilutions. The use of suitable additives to the hemagglutination mixture enables excellent settling patterns and a high sensitivity to be obtained. Direct agglutination, inhibition, enzyme promoted agglutination, and Coombs tests can all be performed. Quantitative studies demonstrate the discriminatory powers of the technic. The method may be useful for economizing in the consumption of reagents in blood grouping.

Từ khóa


Tài liệu tham khảo

Gerke J. R., 1962, Automation of the microbiological assays of antibiotics with an auto‐analyzer instrumental system, Ann. N. Y. Acad. Sci., 93, 625

Gibbs Mary B., 1959, Quantitative immunohematologic studies of hemagglutination: II. Assay of the agglutinogen, A. J. Immun., 82, 577, 10.4049/jimmunol.82.6.577

10.1101/SQB.1964.029.01.044

Sever J. L., 1962, Application of a microtechnique to viral investigations, J. Immun., 88, 320, 10.4049/jimmunol.88.3.320

Takatsy G., 1950, Kiserl, Oviostud. Bes., 2, 393

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Thông tin xuất bản

Transfusion

Tập 6 Số 1

67-73

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