A Recent Outbreak of Human Immunodeficiency Virus Type 1 Infection in Southern China Was Initiated by Two Highly Homogeneous, Geographically Separated Strains, Circulating Recombinant Form AE and a Novel BC Recombinant

Journal of Virology - Tập 74 Số 23 - Trang 11286-11295 - 2000
Sucheep Piyasirisilp1, Francine E. McCutchan2, Jean K. Carr2, Eric Sanders‐Buell2, Wei Liu3, Jie Chen3, Ralf Wagner4, Hans Wolf4, Yiming Shao5, Shenghan Lai6, Chris Beyrer6, Xiaofang Yu7
1Department of Molecular Microbiology and Immunology, The Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205, USA.
2Henry M. Jackson Foundation, Rockville,2 Maryland;
3Guangxi Health and Anti-Epidemic Center, Guangxi,3 and
4Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany4; and
5Chinese Academy of Preventive Medicine, Beijing,5 China
6Department of Epidemiology,6 The Johns Hopkins University School of Hygiene and Public Health, Baltimore, and
7Department of Molecular Microbiology and Immunology1 and

Tóm tắt

ABSTRACT New outbreaks of human immunodeficiency virus type 1 (HIV-1) among injecting drug users (IDUs) are spreading in China along heroin trafficking routes. Recently, two separate HIV-1 epidemics among IDUs were reported in Guangxi, Southern China, where partial sequencing of the env gene showed subtype C and circulating recombinant form (CRF) AE. We evaluated five virtually full-length HIV-1 genome sequences from IDUs in Guangxi to determine the genetic diversity and the presence of intersubtype recombinants. Sequence analysis showed two geographically separated, highly homogeneous HIV-1 strains. B/C intersubtype recombinants were found in three IDUs from Baise City, in a mountainous region near the Yunnan-Guangxi border. These were mostly subtype C, with portions of the capsid and reverse transcriptase (RT) genes from subtype B. The subtype B portion of the capsid was located in the N-terminal domain, which has been shown to influence virus core maturation, virus infectivity, and binding to cyclophilin A, whereas the subtype B portion of RT was located in the palm subdomain, which is the active site of the enzyme. These BC recombinants differed from a BC recombinant found in Xinjiang Province in northwestern China. CRF AE strains were found in IDUs from Nanning, the capital of Guangxi, and in IDUs from Pingxiang City near the China-Vietnam border. The AE and BC recombinants were both remarkable for their low interpatient diversity, less than 1% for the full genome. Rapid spread of HIV-1 among IDUs may foster the emergence of highly homogeneous strains, including novel recombinants in regions with multiple subtypes.

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Journal of Virology

Tập 74 Số 23

11286-11295

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