A Putative MAP Kinase Kinase Kinase,MCK1, Is Required for Cell Wall Integrity and Pathogenicity of the Rice Blast Fungus,Magnaporthe oryzae

Molecular Plant-Microbe Interactions - Tập 21 Số 5 - Trang 525-534 - 2008
Junhyun Jeon1, Jaeduk Goh2, Sungyong Yoo2, Myoung‐Hwan Chi2, Jaehyuk Choi2, Hee‐Sool Rho, Jongsun Park2, Seong-Sook Han3, Byeong Ryun Kim3, Sook‐Young Park4,5, Soonok Kim2, Yong‐Hwan Lee2
1Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Seoul National University, Seoul 151-921, Korea.
2Seoul National Univ.
3Rural development administration
4Pennsylvania State University
5Seoul National University

Tóm tắt

Insertional mutagenesis of Magnaporthe oryzae led to the identification of MCK1, a pathogenicity gene predicted to encode mitogen-activated protein kinase kinase kinase (MAPKKK) homologous to BCK1 in Saccharomyces cerevisiae. Targeted disruption of MCK1 resulted in the fungus undergoing autolysis and showing hypersensitivity to cell-wall-degrading enzyme. The mck1 produced significantly reduced numbers of conidia and developed appressoria in a slightly retarded manner compared with the wild type. Appressorium of the mck1 mutant was unable to penetrate into plant tissues, thereby rendering the mutant nonpathogenic. Cytorrhysis assay and monitoring of lipid mobilization suggested that the appressorial wall was altered, presumably affecting the level of turgor pressure within appressorium. Furthermore, the mck1 mutant failed to grow inside plant tissue. Complementation of the mutated gene restored its ability to cause disease symptoms, demonstrating that MCK1 is required for fungal pathogenicity. Taken together, our results suggest that MCK1 is an MAPKKK involved in maintaining cell wall integrity of M. oryzae, and that remodeling of the cell wall in response to host environments is essential for fungal pathogenesis.

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Molecular Plant-Microbe Interactions

Tập 21 Số 5

525-534

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