A NovelArabidopsis-ColletotrichumPathosystem for the Molecular Dissection of Plant-Fungal Interactions

Molecular Plant-Microbe Interactions - Tập 17 Số 3 - Trang 272-282 - 2004
Richard J. O’Connell1, Corentin Herbert, S. Sreenivasaprasad, Moustafa Khatib, Marie‐Thérèse Esquerré‐Tugayé, Bernard Dumas
1UMR 5546, CNRS-Université Paul Sabatier, Pôle de Biotechnologie Végétale, 24 Chemin de Borde Rouge, BP17 Auzeville, 31326 Castanet-Tolosan, France. [email protected]

Tóm tắt

The ability of a Colletotrichum sp., originally isolated from Brassica campestris, to infect Arabidopsis thaliana was examined. Sequence analysis of the internal transcribed spacer (ITS)1, 5.8S RNA gene and ITS2 regions of ribosomal (r)DNA showed the pathogen to be Colletotrichum destructivum. The host range was broad, including many cruciferous plants and some legumes. At 25°C, all A. thaliana accessions tested were susceptible to the Brassica isolates of C. destructivum; however, at 15°C, the accession Ws-2 showed a temperature-dependant resistance, in which single epidermal cells underwent a rapid hypersensitive response. Legume isolates of C. destructivum were unable to infect A. thaliana and induced deposition of callose papillae at sites of attempted penetration. In compatible interactions, C. destructivum showed a two-stage, hemibiotrophic infection process. The initial biotrophic phase was associated with large, intracellular primary hyphae and was confined to one epidermal cell; whereas, in the subsequent necrotrophic phase, narrow secondary hyphae extensively colonized the tissue and conidia were produced in acervuli. An efficient transformation system was established for C. destructivum, using Agrobacterium-mediated transfer of DNA. The ability to genetically manipulate both partners in the interaction is an important advantage, and the Arabidopsis-Colletotrichum pathosystem should provide a valuable new model for dissecting plant-fungal interactions.

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Tài liệu tham khảo

10.1094/MPMI.1999.12.12.1031

Bailey, J. A., and Jeger, M. J. 1992. Colletotrichum-Biology, Pathology and Control. CAB International, Wallingford, U.K.

10.1016/S0953-7562(09)81382-8

10.1046/j.1365-313X.1996.9060851.x

10.1105/tpc.10.11.1847

10.1094/MPMI.1997.10.6.769

10.1038/72666

10.1016/S0167-7012(02)00234-8

10.1016/S0960-9822(02)00483-9

10.1038/nbt0998-839

10.1094/MPMI.2002.15.10.1040

10.1126/science.266.5188.1247

10.1007/BF02712670

10.1105/tpc.12.9.1579

10.1006/fgbi.1997.1029

10.1046/j.1365-313X.1999.00426.x

10.1139/b86-083

10.2307/3869877

10.1105/tpc.2.6.513

10.1074/jbc.M201489200

Higgins B. B., 1917, J. Agric. Res., 10, 157

10.1094/MPMI-8-0916

10.1094/MPMI-9-0443

10.1105/tpc.2.5.437

Koch E., 1990, Bot. Helv., 100, 257

10.1023/A:1008698113368

10.1016/S0953-7562(96)80226-7

10.3186/jjphytopath.63.395

10.1094/PHYTO.2001.91.2.173

10.1007/PL00000835

10.1016/0048-4059(85)90058-X

10.1111/j.1365-3059.1993.tb01564.x

10.1046/j.1364-3703.2000.00003.x

10.1006/fgbi.1999.1143

10.1046/j.1364-3703.2000.00025.x

10.1094/MPMI.1999.12.11.969

10.1006/emyc.1994.1023

10.1046/j.1365-313x.1998.00319.x

10.1007/s002940050478

10.1046/j.1365-313X.2001.01148.x

Scheffer, R. P. 1950. Anthracnose leafspot of crucifers. N. C. Agric. Exp. Stn. Tech. Bull. 92.

10.1017/S0953756201005111

10.1006/emyc.1994.1014

10.1016/0885-5765(92)90026-R

10.1139/g96-064

Sutton, B. C. 1980. The Coelomycetes: Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Commonwealth Mycological Institute, Kew, U.K.

Sutton, B. C. 1992. The genus Glomerella and its anamorph Colletotrichum. Pages 1-26 in: Colletotrichum-Biology, Pathology and Control. J. A. Bailey, and M. J. Jeger, eds. CAB International, Wallingford, U.K.

Takano Y., 1997, Colletotrichum. Appl. Environ. Microbiol., 63, 351, 10.1128/aem.63.1.351-354.1997

10.1094/PHYTO.2002.92.9.986

10.1046/j.1365-313X.1999.00513.x

10.1016/S0952-7915(00)00183-7

10.1016/S0981-9428(01)01282-7

10.1093/nar/22.22.4673

10.1094/MPMI.2000.13.12.1356

10.1046/j.1365-313X.1997.11061187.x

von Arx J. A., 1957, Phytopathol. Z., 29, 413

10.1094/PHYTO.2001.91.2.149

10.1126/science.291.5501.118

10.1094/MPMI.1999.12.5.430

10.1007/s002940100216

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Molecular Plant-Microbe Interactions

Tập 17 Số 3

272-282

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