A Novel Phytase with Sequence Similarity to Purple Acid Phosphatases Is Expressed in Cotyledons of Germinating Soybean Seedlings

Oxford University Press (OUP) - Tập 126 Số 4 - Trang 1598-1608 - 2001
Carla E. Hegeman1, Elizabeth A. Grabau1
1Department of Plant Pathology, Physiology, and Weed Science, Fralin Biotechnology Center, Virginia Tech, Blacksburg, Virginia 24061–0346

Tóm tắt

Abstract Phytic acid (myo-inositol hexakisphosphate) is the major storage form of phosphorus in plant seeds. During germination, stored reserves are used as a source of nutrients by the plant seedling. Phytic acid is degraded by the activity of phytases to yield inositol and free phosphate. Due to the lack of phytases in the non-ruminant digestive tract, monogastric animals cannot utilize dietary phytic acid and it is excreted into manure. High phytic acid content in manure results in elevated phosphorus levels in soil and water and accompanying environmental concerns. The use of phytases to degrade seed phytic acid has potential for reducing the negative environmental impact of livestock production. A phytase was purified to electrophoretic homogeneity from cotyledons of germinated soybeans (Glycine max L. Merr.). Peptide sequence data generated from the purified enzyme facilitated the cloning of the phytase sequence (GmPhy) employing a polymerase chain reaction strategy. The introduction of GmPhy into soybean tissue culture resulted in increased phytase activity in transformed cells, which confirmed the identity of the phytase gene. It is surprising that the soybean phytase was unrelated to previously characterized microbial or maize (Zea mays) phytases, which were classified as histidine acid phosphatases. The soybean phytase sequence exhibited a high degree of similarity to purple acid phosphatases, a class of metallophosphoesterases.

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Tài liệu tham khảo

Altschul, 1997, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs., Nucleic Acids Res, 25, 3389, 10.1093/nar/25.17.3389

An, 1996, Strong, constitutive expression of Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues., Plant J, 10, 107, 10.1046/j.1365-313X.1996.10010107.x

Bradford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding., Anal Biochem, 72, 248, 10.1016/0003-2697(76)90527-3

Cashikar, 1997, Biochemical characterization and subcellular localization of the red kidney bean purple acid phosphatase., Plant Physiol, 114, 907, 10.1104/pp.114.3.907

Cromwell, 1993, Efficacy of low-activity, microbial phytase in improving the bioavailability of phosphorus in corn-soybean meal diets for pigs., J Anim Sci, 71, 1831, 10.2527/1993.7171831x

Dellaporta, 1983, A plant DNA minpreparation: version II., Plant Mol Biol Rep, 1, 19, 10.1007/BF02712670

Duff, 1994, The role of acid phosphatases in plant phosphorus metabolism., Physiol Plant, 90, 791, 10.1111/j.1399-3054.1994.tb02539.x

Durmas, 1999, The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas): metal content and spectroscopic chracterization., Eur J Biochem, 260, 709, 10.1046/j.1432-1327.1999.00230.x

Felsenstein, 1989, Phylogeny inference package., Cladistics, 5, 164

Finer, 1992, Development of particle inflow gun for DNA delivery to plant cells., Plant Cell Rep, 11, 323, 10.1007/BF00233358

Gibson, 1988, Purification and characterization of phytase from germinating soybean seeds., Arch Biochem Biophys, 260, 503, 10.1016/0003-9861(88)90475-4

Gibson, 1990, Phytases and their action on phytic acid., Inositol Metabolism in Plants., 77

Heinonen, 1981, A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphate., Anal Biochem, 113, 313, 10.1016/0003-2697(81)90082-8

Jackson, 1996, Use of microbial phytase in channel catfish Ictalurus punctatus diets to improve utilization of phytate phosphorus., J World Aquaculture, 27, 309, 10.1111/j.1749-7345.1996.tb00613.x

Klabunde, 1994, The amino acid sequence of the red kidney bean Fe(III)-Zn(II) purple acid phosphatase., Eur J Biochem, 226, 369, 10.1111/j.1432-1033.1994.tb20061.x

Klabunde, 1996, Mechanism of Fe(III)-Zn(II) purple acid phosphatase based on crystal structures., J Mol Biol, 259, 737, 10.1006/jmbi.1996.0354

Koonin, 1994, Conserved sequence pattern in a wide variety of phosphoesterases., Protein Sci, 3, 356, 10.1002/pro.5560030218

Labore, 1993, Purification and characterization of a phytase (myo-inositol-hexakisphosphate phosphohydrolase) accumulated in maize (Zea mays) seedlings during germination., Biochem J, 295, 413, 10.1042/bj2950413

Li, 1997, Secretion of active recombinant phytase from soybean cell-suspension cultures., Plant Physiol, 114, 1103, 10.1104/pp.114.3.1103

Lebansky, 1992, Purification and characterization of a secreted purple phosphatase from soybean suspension cultures., Plant Physiol, 99, 391, 10.1104/pp.99.2.391

Loewus, 2000, Myo-Inositol metabolism in plants., Plant Sci, 150, 1, 10.1016/S0168-9452(99)00150-8

Lohse, 1995, Insights derived from the structures of the Ser/Thr phosphatases calcineurin and protein phosphatase 1., Structure, 3, 987, 10.1016/S0969-2126(01)00234-9

Lott, 1995, Mechanisms and regulation of mineral nutrient storage during seed development., Seed Development and Germination.

Maugenest, 1999, Structure of two maize phytase genes and their spatio-temporal expression during seedling development., Plant Mol Biol, 39, 503, 10.1023/A:1006131506193

Maugenest, 1997, Cloning and characterization of a cDNA encoding a maize seedling phytase., Biochem J, 322, 511, 10.1042/bj3220511

Mitchell, 1997, The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila., Microbiology, 143, 245, 10.1099/00221287-143-1-245

Mullaney, 1998, Identification of a histidine acid phosphatase (phyA)-like gene in Arabidopsis thaliana., Biochem Biophys Res Commun, 251, 252, 10.1006/bbrc.1998.9452

Nielsen, 1997, Identification of prokaryotic and eukaryotic signal pepetides and prediction of their cleavage sites., Protein Eng, 10, 1, 10.1093/protein/10.1.1

Ostanin, 1992, Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase., J Biol Chem, 32, 22830, 10.1016/S0021-9258(18)50022-3

Prattley, 1982, Protein-phytate interactions in soybean: I. Localization of phytate in protein bodies and globoids., J Food Biochem, 6, 243, 10.1111/j.1745-4514.1982.tb00305.x

Raboy, 1997, Accumulation and storage of phosphate and minerals., Cellular and Molecular Biology of Plant Seed Development., 441, 10.1007/978-94-015-8909-3_12

Raboy, 1987, The timing and rate of phytic acid accumulation in developing soybean seeds., Plant Physiol, 85, 841, 10.1104/pp.85.3.841

Ravindran, 1995, Phytates: occurrence, bioavailability and implications in poultry nutrition., Poultry Avian Biol Rev, 6, 125

Reddy, 1989, Phytates in Cereals and Legumes.

Schenk, 1999, Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean., Arch Biochem Biophys, 370, 183, 10.1006/abbi.1999.1407

Schenk, 2000, Identification of mammalian-like purple acid phosphatases in a wide range of plants., Gene, 250, 117, 10.1016/S0378-1119(00)00186-4

Sharpley, 1994, Managing agricultural phosphorus for protection of surface waters: issues and options., J Environ Qual, 23, 437, 10.2134/jeq1994.00472425002300030006x

Shieh, 1968, Survey of microorganisms for the production of extracellular phytase., Appl Microbiol, 16, 1348, 10.1128/am.16.9.1348-1351.1968

Simons, 1990, Improvement of phosphorus availability by microbial phytase in broilers and pigs., Br J Nutr, 64, 525, 10.1079/BJN19900052

Sträter, 1995, Crystal structure of a purple acid phosphatase containing a dinuclear Fe(III)-Zn(II) active site., Science, 268, 1489, 10.1126/science.7770774

Sutardi, 1986, The characteristics of soybean phytase., J Food Biochem, 10, 197, 10.1111/j.1745-4514.1986.tb00100.x

Thompson, 1994, CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice., Nucleic Acids Res, 22, 4673, 10.1093/nar/22.22.4673

Totsuka, 1993, Expression and mutation of soybean beta-amylase in Escherichia coli., Eur J Biochem, 214, 787, 10.1111/j.1432-1033.1993.tb17981.x

Ullah, 1993, Aspergillus ficuum phytase: complete primary structure elucidation by chemical sequencing., Biochem Biophys Res Commun, 192, 747, 10.1006/bbrc.1993.1477

Ullah, 1988, Purification and characterization of acid phosphatase from cotyledons of germinating soybean seeds., Arch Biochem Biophys, 260, 514, 10.1016/0003-9861(88)90476-6

Wodzinski, 1996, Phytases., Adv Appl Microbiol, 42, 263, 10.1016/S0065-2164(08)70375-7

Yi, 1996, Improving phytate phosphorus availability in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase., Poultry Sci, 75, 240, 10.3382/ps.0750240

Zhou, 1994, Mutational analysis of a Ser/Thr phosphatase., J Biol Chem, 269, 26234