A Novel Multiplex Real-Time PCR Assay for Rapid Typing of Major Staphylococcal Cassette Chromosome <i>mec</i> Elements

Journal of Clinical Microbiology - Tập 42 Số 7 - Trang 3309-3312 - 2004
Patrice François1, Gesuèle Renzi2, Didier Pittet3, Manuela Bento1, Daniel Lew2,1, Stephan Harbarth3, Pierre Vaudaux1, Jacques Schrenzel2,1
1Genomic Research Laboratory
2Clinical Microbiology Laboratory, Division of Infectious Diseases
3Infection Control Program, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland

Tóm tắt

ABSTRACT We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

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Thông tin xuất bản

Journal of Clinical Microbiology

Tập 42 Số 7

3309-3312

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