A Method for Measurement of Cholesterol in Blood Serum

A method for measuring cholesterol in 0.1 ml. of blood serum is described. This uses the saponification and extraction procedure of Abell et al. (4) and the color reaction of Zlatkis et al. (7). The procedure is shown to be accurate and reproducible. It is compared with several commonly used procedures and shown to be equal or superior to these. An adaptation of the method to filter-paper stored samples described. The method is recommended for use in clinical laboratories.